猪PAC克隆的微卫星DNA分离研究  

Studies of Microsatellite Isolation from Porcine PAC Clone

作　　者：高军[1] 任军[1] 陈克飞[1] 黄路生[1] Bertram Brenig 

机构地区：[1]江西农业大学江西省动物生物技术重点实验室,南昌330045 [2]德国哥廷根大学兽医研究所,哥廷根37073

出　　处：《遗传》2003年第6期660-662,共3页Hereditas（Beijing)

基　　金：国家自然科学基金"江西地方猪16个候选基因分子变异与种质资源评估研究"资助(30060058)

摘　　要：利用(CA)8核心序列,设计锚定引物,采用直接测序法,从单个猪PAC克隆中分离到一个新微卫星DNA。根据该微卫星DNA的侧翼序列,设计了专一引物,在8个猪种40个个体中检测到3个等位基因,片段长度分别为305bp、307bp和309bp。3种等位基因纯合子个体的PCR产物序列分析表明,这3种等位基因分别有12、13和14次CA双核苷酸重复。A novel microsatellite DNA was isolated from a single porcine PAC clone by sequencing the PAC clone directly with (CA)n repeat motif anchored primer.The specific primer pairs flanking the (CA)n repeat region were used to amplify the genomic DNA of 40 individuals from 8 pig breeds,which detected three alleles with the fragment length of 305 bp,307 bp and 309 bp.The PCR product sequencing results of homozygous animals representing three alleles revealed that those three alleles contained 12,13 and 14 CA dinucleotide repeats respectively.

关 键 词：猪 PAC克隆 微卫星DNA 锚定引物 

分 类 号：Q785[生物学—分子生物学]