小鼠超数排卵影响因素研究  

Study on Impact Factors of Mice Superovulation

作　　者：楼吉 唐业刚[2] 周骏江[2] 郭云贵[2] 邓家庆[2] 

机构地区：[1]上海泽润生物科技有限公司生产部 [2]武汉生物工程学院生物工程系

出　　处：《武汉生物工程学院学报》2009年第1期18-20,43,共4页Journal of Wuhan Bioengineering Institute

摘　　要：从小鼠发情周期、小鼠日龄及注射超排激素剂量3个方面对昆明白小鼠超数排卵的效果进行了研究。结果表明:小鼠发情初期与发情间期对超排的效果差异不显著(P】0.05),35日龄的小鼠超排效果显著低于(P【0.05)60、90日龄的小鼠,孕马血清促性腺激素(PMSG)(10 IU)+人绒毛膜促性腺激素(hCG)(10 IU)剂量组合超排效果极显著高于(P【0.01)其他3组剂量[PMSG(5 IU)+hCG(5 IU)、PMSG(7.5 IU)+hCG(7.5 IU)、PMSG(12.5 IU)+ hCG(12.5 IU)]组合。输卵管全段剖开法是本实验条件下收集早期胚胎的一种可行方法。Three factors(estrous cycle,day-age and injection dose of superovulation hormone) affecting superovulation of Kunming white mice were studied.The results indicated that the effect of superovulation had no significant difference between proestrus group and diestrus group(P>0.05),superovulation of 35 day-age mice group was significantly lower than that of 60 day-age and 90 day-age mice groups(P<0.05),and superovulation of PMSG(10 IU)+hCG(10 IU)group was very significantly higher than that of the other three groups:PMSG(5 IU)+hCG(5 IU),PMSG(7.5 IU)+hCG(7.5 IU),and PMSG(12.5 IU)+hCG(12.5 IU)(P<0.01).Moreover,the experiment also demonstrated that segmenting the whole oviduct was a feasible way to collect early embryos under this experimental condition.

关 键 词：小鼠超数排卵 小鼠日龄 孕马血清促性腺激素(PMSG) 人绒毛膜促性腺激素(hCG) 

分 类 号：Q813[生物学—生物工程]