Box-Behnken响应面法优化芦笋总皂苷超声提取工艺  

Optimization of Ultrasound Extraction Technology for Total Saponins from Asparagus officinalis by Box-Behnken Response Surface Methodology

作　　者：揭晶[1] 赵越[2] 王皓 

机构地区：[1]武汉生物工程学院药学院 [2]广东药学院中药学院

出　　处：《武汉生物工程学院学报》2015年第4期293-296,300,共5页Journal of Wuhan Bioengineering Institute

基　　金：湖北省自然科学基金资助项目(2012FFB05201);湖北省高等学校青年教师深入企业行动计划资助项目(XD2012501)

摘　　要：优选芦笋总皂苷的超声提取工艺,以总皂苷含量为指标,在单因素试验基础上,通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对提取工艺的影响。最佳超声提取工艺为加15倍量74%乙醇于50℃超声提取54 min;总皂苷平均质量分数13.059%(RSD 1.63%),与预测值(13.185%)的偏差较小。优选的提取工艺稳定可行,为芦笋总皂苷的开发应用提供实验依据。To optimize the ultrasound extraction technology of the total saponins from Asparagus officinalis,with the content of total saponins as index,the effects of ethanol concentration,solid-liquid ratio,ultrasonic time and temperature on the extraction technology were investigated by single factor test and Box-Behnken response surface methodology.The results showed that the optimum ultrasound extraction technology was as following:ultrasonic extracted 54 min.with 15 times the amount of 74%ethanol at50 ℃;Under these conditions,the average mass fraction of total saponins was 13.059%,which was very close to the predicted value of 13.185%.This optimized technology was stable and feasible to provide a basis for development and application of total saponins in A.officinalis.

关 键 词：芦笋 总皂苷 超声提取工艺 单因素试验 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学]