深圳塘朗山桫椤孑遗种群的遗传分化研究  

Studies on the Genetic Differentiation of a Relict Population of Alsophila spinulosa in Tanglangshan Mountain, Shenzhen, China

作　　者：苏应娟[1] 王艇[1] 李雪雁[1] 陈国培[1] 江宇[1] 欧阳蒲月[1] 郑博[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2003年第6期69-72,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30170101);国家自然科学基金重点资助项目(39830310);广东省自然科学基金资助项目(011125)

摘　　要：应用RAPD标记分析了深圳塘朗山桫椤孑遗种群全部57个个体的遗传变异和进化关系。50个引物共检测到171个位点,其中多态位点10个,多态位点比率5 85%。基于基因频率的Shannon多样性指数平均值为0 0165,Nei基因多样度指数平均值为0 0094,表明该种群的遗传多样性水平极低。对Jaccard相似性系数矩阵用UPGMA法进行聚类分析显示,57个个体可分为4个亚群。RAPD谱带表型的主成分分析(PCA)支持聚类分析结果。根据研究结果讨论了种群的管理和保护策略。RAPD markers were used to assess the genetic variations and the evolutionary relationships of all 57 individuals of a relict population of Alsophila spinulosa naturally grown in Tanglangshan mountain, Shenzhen, South China. 50 random primers detected 171 sites of which 10 ones were polymorphic, the percentage of polymorphic sites was 5.85%. Average values of index of Shannon diversity based on gene frequencies and Nei's index of gene diversity were 0.016 5 and 0.009 4, respectively, which demonstrated that the relict popuplation possesses very low level of genetic diversity. Cluster analysis of the Jaccard coefficient matrix using UPGMA method revealed that 57 individuals were clustered into 4 subpopulations which was also lent support from the spatial representation of principle components analysis (PCA) of RAPD phenetic patterns. Management and protection strategy of the relict population was discussed based on these results.

关 键 词：桫椤 种群 RAPD标记 遗传变异 孑遗种群 进化 遗传分化 基因 

分 类 号：Q941[生物学—植物学] Q948