红螯螯虾感光器中Gq蛋白的鉴定及光波长对其含量的影响  

Identification of the Gq-protein in Cherax quadricar-inatus Photoreceptor and the Influence of Wave- length Light on the Content of Gqα

作　　者：许燕[1] 袁维佳[1] 赵云龙[2] 徐静[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]华东师范大学生命科学学院,上海200062

出　　处：《Zoological Research》2003年第6期429-434,共6页动物学研究（英文）

摘　　要：用蛋白质常规提取方法分别提取红螯螯虾复眼视网膜的可溶性和膜结合蛋白质 ,用SDS PAGE和免疫印迹法进行G蛋白的分离和鉴定 ;在经光、暗适应及红、绿、蓝、黄 4种波长光照处理后 ,对其感光器中G蛋白分别进行SDS PAGE分析。暗适应、自然光及 4种波长光适应的感光器可溶性蛋白、膜结合蛋白条带数量和分子量大小基本相同 ,在 4 2kD处都出现一条清晰的蛋白条带。兔抗Gαq 11多克隆抗体能识别这一蛋白条带。表明红螯螯虾感光器中存在可溶性和膜结合Gq蛋白α亚基 ,分子量为 4 2kD。在不同光照条件下感光器中可溶性和膜结合Gqα亚基的含量有差异。可溶性Gqα蛋白占总蛋白的百分含量依次为 :日光 (7 71% ) >黄光(7 32 % ) >红光 (7 0 6 % ) >暗适应 (6 94 % ) >蓝光 (6 4 6 % ) >绿光 (5 74 % ) ;除暗适应与红光之间差异不显著外 ,红光与黄光差异显著 ,其余差异极显著。而膜结合Gqα亚基的百分含量则相反 ,依次为 :绿光(13 94 % ) >蓝光 (10 5 6 % ) >暗适应 (10 2 5 % ) >红光 (10 14 % ) >黄光 (9 76 % ) >日光 (9 4 4 % ) ;除暗适应与红光差异不显著外 ,红光与黄光差异显著 ,其余差异极显著。推测红螯螯虾感光器中Gq蛋白的激活与光照刺激的波长有关 ,其感光器对不同波长光刺激的敏感度有差别。The soluble and membrane-bound protein in the photoreceptor of the Cherax quadricarinatus,which were extracted by common method of protein-extraction,respectively,were separated and identified with SDS-PAGE and western blotting.After dark adaptation and the illumination by sun-light and four kinds of different wavelength lights (red,green,blue,yellow),the content of the G-protein in the photoreceptor of C.quadricarinatus was analyzed with SDS-PAGE.The result of SDS-PAGE indicated:the numbers and the molecule mass of the protein bands in soluble and membrane fractions of the photoreceptor under different light conditions were basically similar,there was an apparent protein band at 42?kD.The results of western blotting showed that anti-Gqα antibodies recognized the 42?kD protein not only in the photoreceptor membrane but also in soluble fractions of the retina,the G-protein in the photoreceptor of C.quadricarinatus is a Gq-class G-protein.The content of two forms of Gqα,soluble Gqα and membrane-bound Gqα,changed depending on the light condition.The contents of the soluble Gqα were in turn,sun-light (7.71%)> yellow-light(7.32%)>red-light (7.06%)> dark adaptation(6.94%)> blue-light (6.46%)>green-light (5.74%);There was significantly different between red-light and yellow-light,very significantly different among others,except between dark-adapted and red-light.The content of the membrane-bound Gqα was contrary to the soluble Gqα.The contents of the membrane-bound Gqα were in turn,green-light (13.94%)> blue-light (10.56%)> dark-adapted (10.25%)> red-light (10.14%)> yellow-light (9.76%)> sun-light (9.44%);There was also significantly different between red-light and yellow-light,very significantly different among others,except between dark-adapted and red-light.These results indicate that the activation of the Gq-protein in the photoreceptor of C.quadricarinatus depends on the lights of different wavelength.The light sensitivity of the photoreceptor was different under different wavelength lights.

关 键 词：红螯螯虾 感光器 Gq蛋白 光波 

分 类 号：Q51[生物学—生物化学]