饵料维生素E含量对酸应激中华鳖幼鳖血清补体C3和C4的影响  

Effects of Dietary Vitamin E on Contents of Serum Complement C3 and C4 in Acid Stressed Juvenile Soft-shelled Turtles (Pelodiscus sinensis)

作　　者：周显青[1] 牛翠娟[1] 孙儒泳[1] 

机构地区：[1]北京师范大学生命科学学院

出　　处：《Zoological Research》2003年第6期452-456,共5页动物学研究（英文）

基　　金：中国科学院水生生物研究所淡水生态和生物技术国家重点实验室开放课题资助~~

摘　　要：为探讨维生素E (VE)对中华鳖 (Pelodiscussinensis)幼鳖血清补体C3和C4的影响及补体在酸应激下的变化 ,在 6组 (对照组 ,实验Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ组 )幼鳖的饵料中依次添加 0、 5 0、 2 5 0、 5 0 0、 10 0 0和5 0 0 0mg/kg的VE,喂食 4周 ,每组取半数幼鳖经酸应激处理 2 4h。取幼鳖血清 ,用透射比浊法测定血清补体C3和C4的含量。经和未经酸应激的实验Ⅱ、Ⅲ和Ⅳ组幼鳖血清补体C3的含量均明显高于对照组 ,实验Ⅰ和Ⅱ组C4含量也明显高于对照组 ;经酸应激的幼鳖与未经酸应激的比较 ,对照组和实验Ⅰ组C3和C4的含量显著下降 ,其余 4组没有变化。分析说明 ,VE 在一定剂量范围内能促进血清补体C3和C4的合成 ,酸应激能导致其下降 ;而高剂量的VE 对酸应激导致的不利影响有拮抗作用。To investigate the effect of dietary vitamin E (V E) on serum complement C3 and C4,and the change of the complement under acid-stress in juvenile soft-shelled turtles (Pelodiscus sinensis),total 72 animals of one control group (C) and five experimented groups (Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ) were fed with V E supplementation at the dosages of 0,50,250,500,1?000 and 5?000?mg/kg diets,respectively.After feeding 4 weeks,half turtles per group were treated with acid stress for 24?h,and then the levels of their serum complement C3 and C4 were analyzed by transmission colorimetric method.The results showed that C3 levels in Ⅱ,Ⅲ and Ⅳ were significantly higher than that of C,and C4 levels in Ⅰ and Ⅱ were distinctly higher than that of C;C3 and C4 levels of both C and Ⅰ in stressed turtles had significant decreases compared with non-stressed ones,while the other four groups had no significant changes.Those suggest that V E seems to have an upper and lower threshold for improving levels of serum complement C3 and C4 in juvenile turtles,and acid stress decreases the levels,while the high dosage V E can withstand this decline.

关 键 词：中华鳖 维生素E 酸应激 补体C3 补体C4 

分 类 号：S963[农业科学—水产养殖] S966.5[农业科学—水产科学]