水飞蓟宾脂质体包封率及其水飞蓟宾含量测定  

Determination of Concentration and Encapsulation Efficie ncy of Silybin Liposomes

作　　者：余江南[1] 徐希明[1] 朱源[1] 高昊[1] 

机构地区：[1]江苏大学药学院,江苏镇江212001

出　　处：《中国中药杂志》2003年第11期1027-1030,共4页China Journal of Chinese Materia Medica

基　　金：江苏省自然科学基金资助项目 (BK 2 0 0 2 0 0 7);江苏高校高新技术产业化项目 (JH 0 1 0 6 1 )

摘　　要：目的 :建立一种一阶导数紫外分光光度法测定水飞蓟宾脂质体包封率及脂质体中水飞蓟宾含量。方法 :水飞蓟宾脂质体溶液经SephadexG10 0 柱分离后 ,以水飞蓟宾一阶导数紫外扫描图谱在 2 77nm和 2 94nm处出现的特征峰和特征谷的振幅ΔA进行定量。结果 :方法的线性范围为 0 .5～ 3 0 .0mg·L- 1(r =0 .9998) ,回收率为10 1.7% ,精密度为 1.8%。采用该法测定药脂比为 0 .0 2～ 0 .0 6的水飞蓟宾脂质体 ,包封率为 65.1%～ 83 .0 % ,脂质体中水飞蓟宾平均含量为 760 .4mg·L- 1(n =4)。结论 :一阶导数分光光度法准确、简便 。Objective: To determine the encapsulation efficiency of silybin lipos omes. Method: After Silybin liposomes solution was separat ed through sphadex G- 100 column, eluent collected was determined by the first derivative UV spectrome try. Result: The calibration curve of Silybin was linear i n the range of 0.5～30 .0 mg·L -1 (r=0.999 8).The average recovery was 101.7%. The relat ive standard de viation was 1.8%. The results showed that the encapsulation efficiency ranged fr om 65.1%～83.0% when the drug /phospholipid ratio varied from 0.02～0.06. The co ncentration of SLB in liposomes prepared by optimum technique was 760.4 mg·L -1 ( n=4). Conclusion: The method is accurate, simple and s uitable for the quality control of Silybin liposomes.

关 键 词：水飞蓟宾脂质体 包封率 含量 测定 一阶导数分光光度法 

分 类 号：R284[医药卫生—中药学]