芦荟抗菌作用与蒽醌化合物的关系  

Relationship Between Antibacterial Activity of Aloe and Its Anthaquinone Compounds

作　　者：田兵[1] 华跃进[1] 马小琼[2] 王关林[3] 

机构地区：[1]浙江大学应用生物科学系原子核农业科学研究所,浙江杭州310029 [2]中国科学院化学物理研究所,辽宁大连116012 [3]辽宁师范大学生物工程研究所,辽宁大连116029

出　　处：《中国中药杂志》2003年第11期1034-1037,共4页China Journal of Chinese Materia Medica

摘　　要：目的 :分析芦荟抗菌作用与蒽醌化合物含量的关系 ,比较芦荟大黄素和芦荟苷的抗菌作用 ,探讨糖基取代结构对芦荟苷抗菌活性的影响。方法 :利用琼脂扩散法分析皂质芦荟叶皮提取物、芦荟大黄素和芦荟苷对 3种革兰氏阴性菌、2种革兰氏阳性菌的抑菌活性 ,利用扫描电镜技术分析了芦荟苷对大肠杆菌的作用。结果 :芦荟的抗菌作用与蒽醌化合物的含量呈正相关性。芦荟苷 (1g·L- 1)具有较强的抗菌活性 [抑菌直径大于 (7.1± 0 .15)mm] ,对革兰氏阳性菌的抗性高于革兰氏阴性菌。芦荟大黄素的抗菌活性较弱。芦荟苷能够改变细胞的形态 ,破坏细胞结构。结论 :蒽醌化合物是芦荟中的抗菌成分 ,芦荟苷是主要抗菌有效成分之一 。Objective: To investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone comp ounds, measure and compale antibacterial activities of aloin and aloe-emodin, a nd analyse the effect of glycoside on the antibacterial activity of aloin. Method: The antibacterial activities of the extracts from the o uter leaf of Aloe saponaria Haw, aloin and aloe-emodin against three Gram-nega tive and two Gram-positive bacteria were investigated with the method of agar d iffusion. The antibacterial effect of aloin on E. coli was further studied with scanning electron microscopy. Result: The antibacterial ac tivities of aloe showed to be dependent on the dose of anthraquinone, aloin (1 g ·L -1 ) exhibited higher antibacterial activity [inhibition diameter>(7.1 ±0.15) mm] than Aloe-emodin (inhibition diameter<5.0 mm), and aloin changed t he morphology of E. coli and damaged the outer cell structrue. Concl usion: Anthraquinone compounds are the active antibacterial component s in aloe and aloin is the main active compound. The glycoside makes it easy for aloin to invade cells and enhances its activity.

关 键 词：芦荟 抗菌作用 蒽醌化合物 芦荟苷 芦荟大黄素 

分 类 号：R285[医药卫生—中药学]