2001年广州市婴幼儿杯状病毒腹泻病的基因研究  

Genotype of human calicivirus from infants with diarrhea in Guangzhou city in 2001

作　　者：谢健屏[1] 方肇寅 龚四堂[3] 朱冰[1] 区文玑[3] 万根平[1] 赖桂香[1] 何翠娟[1] 

机构地区：[1]广州市儿童医院中心实验室,510120 [2]中国预防科学院病毒研究所 [3]广州市儿童医院内科,510120

出　　处：《中华儿科杂志》2003年第11期842-844,共3页Chinese Journal of Pediatrics

基　　金：广东省卫生厅资助项目 (WST112 0 0 0 0 10 6)

摘　　要：目的　了解广州地区婴幼儿腹泻病杯状病毒感染的特点及病毒的基因型。方法　收集 2 0 0 1年 11～ 12月因非细菌性腹泻病而来我院门诊或住院的患儿粪便共 2 2例。使用RT PCR方法对大便标本进行杯状病毒检测。杯状病毒RT PCR阳性的标本分别克隆到PMD18 T载体中后 ,转化进入大肠杆菌DH5a感受态细胞 ,通过蓝、白斑挑选克隆 ,用限制性内切酶EcoRI和BamHI筛选克隆 ,用PCR鉴定阳性克隆。序列测定由宝生物工程 (大连 )公司完成。结果　 2 2例标本中杯状病毒RT PCR阳性 2例 ,杯状病毒阳性率 9% (2 / 2 2 )。测序位置为RNA多聚酶片段。序列分析结果为诺沃克样病毒基因 2型 ,与北京CR84 0共享序列非常接近。结论　杯状病毒是广州地区婴幼儿腹泻病的病原之一。基因分析为诺沃克样病毒基因 2型。Objective To understand the characteristics of human calicivirus (HuCV) infection in infants with diarrhea in Guangzhou city and to study genotype of the virus.Methods The authors collected fecal specimens from 22 children with acute nonbacterial gastroenteritis from November to December, 2001. HuCV was detected from the specimens by RT-PCR. The PCR products were cloned into the PMD18-T cloning vector and sequenced. Results HCV was detected from the specimens of 2 cases (9%, 2/22). The nucleotide sequence analysis revealed that the virus strains belonged to genotype 2 of Norwalk-like viruses.Conclusion HuCV is one of the pathogens causing diarrhea in infants and young children in Guangzhou area. HuCV infection occurred sporadically in autumn and winter.

关 键 词：广州市 婴幼儿 杯状病毒 腹泻 基因研究 

分 类 号：R725.1[医药卫生—儿科]