反相高效液相色谱法测定血浆中的辅酶Q_(10)  

Determination of Coenzyme Q_(10) in Human Plasma by Reversed-Phase High Performance Liquid Chromatography

作　　者：江平[1] 辛剑[1] 郑育芳[2] 吴美慧[3] 许国旺[2] 

机构地区：[1]大连理工大学化工学院,辽宁大连116023 [2]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116011 [3]大连市中心医院,辽宁大连116033

出　　处：《色谱》2003年第6期590-592,共3页Chinese Journal of Chromatography

基　　金：国家科技部国家高技术研究发展计划(2003AA223061);中国科学院知识创新工程领域前沿课题.

摘　　要：建立了一种检测血浆中辅酶Q10含量的高效液相色谱法。血浆经甲醇脱脂蛋白后,以正己烷萃取,萃取液依次经硅胶柱净化、C18柱固相萃取,再进行高效液相色谱分析。色谱柱为HypersilODS2柱(5μm,150mm×4 6mmi d ),以异丙醇 甲醇(体积比为45∶55)溶液作流动相,辅酶Q9作内标,检测波长为275nm。在0 1～50 0mg/L质量浓度范围内,辅酶Q10与辅酶Q9的峰面积比与相应CoQ10的质量浓度呈良好的线性关系(r2=0 999),血浆中辅酶Q10的检测限为0 03mg/L(S/N=3),加标回收率为96%～98%,方法重现性好(RSD<3%)。用此法测定正常人血浆中辅酶Q10的含量水平,结果令人满意。An improved high performance liquid chromatographic (HPLC) method for the determination of coenzyme Q10(CoQ10) in human plasma has been developed. CoQ10 was dissociated from lipoproteins in plasma by treatment with methanol and extracted with nhexane, then cleanedup on silica gel and C18 solidphase extraction cartridges and finally analyzed by HPLC. The HPLC conditions were: A Hypersil ODS2 column (5 μm, 150 mm×46 mm id) as analytical column, isopropanolmethanol (45∶55, v/v) at a flowrate of 1 mL/min as mobile phase, coenzyme Q9 (CoQ9) as internal standard and UV 275 nm as detection wavelength. The linearity between concentration and peak area ratio (CoQ10/CoQ9) was in the range from 01 to 500 mg/L (r2=0999) and the minimum detectable CoQ10 plasma level was 003 mg/L (S/N=3). The recoveries ranged from 96% to 98%. The method has a good reproducibility with RSD less than 3%. Finally, the method was applied to determine CoQ10 concentrations in healthy human plasma.

关 键 词：反相高效液相色谱法 测定 血浆 辅酶Q10 固相萃取 疾病诊断 

分 类 号：R446.112[医药卫生—诊断学]