高效液相色谱法测定红花中的羟基红花黄色素A  

Determination of Hydroxy Safflower Yellow A in Carthamus tinctorius L. by High Performance Liquid Chromatography

作　　者：赵明波[1] 邓秀兰[2] 王亚玲[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院中医药现代研究中心,北京100083 [2]北京中医药大学基础医学院,北京100029

出　　处：《色谱》2003年第6期593-595,共3页Chinese Journal of Chromatography

基　　金：国家经贸委中药材生产扶植资金项目(国经贸医药200056).

摘　　要：建立了菊科植物红花中的主要有效成分羟基红花黄色素A的高效液相色谱定量分析方法。用90℃水提取,以甲醇 0 5%磷酸水溶液(体积比为40∶60)为流动相,检测波长400nm。该方法的最低检出限为4ng(按S/N=3计)。在羟基红花黄色素A的质量浓度为0 04～0 40g/L(相当于绝对进样量为0 8～8 0μg)时线性良好,方法回收率高,重现性好。对26个不同产地和购买地的红花中的羟基红花黄色素A进行了测定,结果表明不同来源的红花中羟基红花黄色素A含量的差异较大。Hydroxy safflower yellow A in Carthamus tinctorius L. (a Chinese traditional medicine) was determined by reversedphase high performance liquid chromatography (RPHPLC) with UV detector at 400 nm. Samples were extracted with water at 90 ℃. A Phenomenex Luna column, 250 mm×46 mm id and a mobile phase of methanol05% phosphoric acid (40∶60, v/v) were used for the assay. The calibration curve of hydroxy safflower yellow A was linear in the range of 08-80 μg. The correlation coefficient was 0999?98. Twentysix samples from different purchase sites or habitats were assayed and the results show that the contents of hydroxy safflower yellow A in Carthamus tinctorius L. varied from 060%-265%.

关 键 词：高效液相色谱法 测定 红花 羟基红花黄色素A 中药 有效成分 

分 类 号：R284.1[医药卫生—中药学] R282.5[医药卫生—中医学]