HCV抗原诱导Th1/Th2细胞增殖活性分析及其在丙型肝炎诊断中的应用  

Analysis of HCV antigen inducing the proliferating activation of Th1 and Th2 cells and research on its clinical significance

作　　者：宋玉国[1] 张吉林[1] 黄建文[1] 武玉久[1] 毕胜利[1] 

机构地区：[1]北华大学医学院附属医院临床免疫研究室,吉林吉林132011

出　　处：《中国实验诊断学》2003年第6期480-483,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省教育厅科研基金资助课题;吉教合字 ( 0 0 )第 2 8号

摘　　要：目的　通过检测Th1 Th2特征性细胞因子 ,分析Th1 Th2细胞增殖活性 ,探讨其在丙型肝炎慢性化进程中的免疫学作用 ,研究HCV感染者肝病慢性化的免疫学因素。方法　首先用淋巴细胞分离液分离丙肝病毒感染者的PB MC ,纯化其CD+4T细胞 ,然后用HCV混合Ag刺激培养CD+4T细胞 ,最后用ELISA法检测IL 2、IL 4的含量 ,分析Th1和Th2细胞增殖活性。结果　本课题研究病例共包括 2 3例急性肝炎病例 (其中 7例呈自限性病程 ,16例转变为慢性肝炎 )和 2 0例慢性肝炎病例 ,以上病例CD+4T细胞经HCV混合抗原刺激培养后 ,其中 7例急性自限性病例表现为Th1样反应 (IL 2分泌增加 ) ,与 16例急性转慢性病例比较有显著性差异 (t =2 1.76、P <0 .0 1) ;7例自限性病例在HCVAg刺激前后Th1样反应有显著性差异 (t =18.15、P <0 .0 1)。 16例急性转慢性病例表现为Th2样反应 (IL 4分泌增加 ) ,与 7例自限性病例比较有显著性差异 (t =7.75、P <0 .0 1) ;16例急性转为慢性病例在HCVAg刺激前后Th2样反应有显著性差异 (t=4 .0 8、P<0 .0 1)。 2 0例慢性化病例主要为Th2样反应 ,其与急转慢性病例无显著性差异 (t=1.0 1、P >0 .0 5 )。结论　HCVAg能够特异性地刺激Th前体细胞向Th1或Th2细胞分化 ;Th1样应答与疾病的好转有关 ,Th2样应答与慢性化有?Objective A analyses proliferating activation of Th1 and TH2 cell through testing Th1/Th2 specialized cytokines, make an inquiry into its immune function in process of hepatitis C chronicity and study immune factors of HCV infection chronic-city.Methods Separate PBMC of hepatitis C with lymphocytes separating fluid, purify its CD+ 4 T cells with high affinity negative selecting method. Stimulate and cultivate purified CD+ 4 T cell by HCV antigen. Measure the concentration of IL-2 and IL-4 with ELISA and analyses proliferating activation of Th1 and Th2 cell.Results There were 23 patients with hepatitis C (7 patients with self-limited disease and 16 patients with chronic evolution) and 20 patients with chronic hepatitis C in this research. Purified CD+ 4T cells of all the patients were stimulated by HCV antigen. Of the 23patients with acute hepatitis C, 7 patients with self-limited disease presented Th1-like reponses (increase of IL-2 secretion), which was different from the other 16 patients (t=21.76,P<0.01).And before HCV-antigen stimulating and after that, there was a significant difference in the Th1-like response(t=18.15,P<0.01) of 7 patients with self-limited disease. 16 acute patients with chronic evolution appeared Th2-like responses (increase of IL-4 secretion), which was different from other 7 patients, (t=7.75,P<0.01). There was also a significant difference in the Th2-like responses before and after HCV-antigen stimulating (t=4.08,P<0.01). All of the 20 patients with chronic hepatitis C presented Th2-like reponses. While there was no significant difference in Th2-like responses compared with those of acute patients with chronic evolution (t=1.01,P>0.05).Conclusion HCV antigen can specifically stimulate the differentiation of precursor T-helper cells into both Th1 cells and Th2 cells. There was correlation between Th1-like responses and recovering from hepatitis C. There was correlation between Th2-like responses and chonicity of acute hepatitis C.

关 键 词：HCV抗原 诱导 TH1/TH2 细胞增殖活性 丙型肝炎 诊断 

分 类 号：R512.63[医药卫生—内科学]