电泳法测定碱性磷酸酶同工酶的临床意义  

Clinical Significance of Alkaline Phosphatase Isoenzymes examination by Agarose Electrophoresis

作　　者：孙淑艳[1] 梁娜[2] 艾清[1] 

机构地区：[1]吉林大学第一医学院检验科,吉林长春130021 [2]吉林大学第一医学院,本科硕士研究生吉林长春130021

出　　处：《中国实验诊断学》2003年第6期487-489,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨碱性磷酸酶同工酶测定的临床意义。方法　用神经氨酸酶孵育血清快速电泳法检测 181例血清ALP同工酶。结果　健康成人ALP同工酶电泳主要为肝型和骨型 ,其所占比例分别为 34.5 %～ 6 2 .3%和 37.7%～6 5 .5 % .男女性别之间ALP无明显差异 (P >0 .0 5 ) .儿童ALP同工酶主要为骨型ALP ,占 85 %以上 .肝、骨ALP分离彻底 ,区带清晰。肝病 :主要为肝型ALP ,达 80 %以上 ;骨病 :主要为骨型ALP ,达 80 %以上 ;阻塞性黄疸 :为肝型和骨型ALP活性均增高 ,但以肝型ALP增高更为突出 ;佝偻病 :为骨型ALP轻、中度升高 ,不超过总活性的 70 % ;孕妇 :在肝和骨之间出现胎盘型ALP峰。结论　测定ALP总活性及其同工酶对引起ALP活性增高疾病的诊断和鉴别诊断有重要的临床意义。Objective To inquire the significance of quantifying the Alkaline Phosphatase(ALP)Isoenzymes by Agarose Electrophoresis.Methods Use a fast Electrophoresis method of detecting theALP Isoenzymes by using preincubated sera with neuraminidase before Electrophoresis to detect 181 cases human serum ALP Isoenzymes.Results The activity of total ALP and ALP Isoenzymes were assayed in 181 cases sera(included 39 normal adults ,22 normal childen ,and 118 patients with liver disease ,bone disease, obstruction jaundice and gravidas),the result showed that liver ALP increased in liver disease;bone ALP increased in bone disease;and in gravida a placenta zone was showed beteen liver ALP and bone ALP;but,in obstruction jaundice both liver ALP and bone ALP were increased`.Conclusion quantifying the tatol activity of ALP and it`s Isoenzymes by Agarose Electrophoresis have importance in diagnosis and distinguish for all diseases leading to ALP increased.

关 键 词：电泳法 测定 碱性磷酸酶 同工酶 神经氨酸酶 唾液酸 

分 类 号：R446.1[医药卫生—诊断学]