钩端螺旋体DNA疫苗pcD-flaB在豚鼠体内的免疫保护性研究  

DNA vaccine pcD-flaB protects guinea pigs from Leptospira infection

作　　者：寇志华[1] 陈祖欢[1] 孙树汉[1] 郭瀛军[1] 张平武[1] 

机构地区：[1]第二军医大学医学遗传学教研室

出　　处：《中华微生物学和免疫学杂志》2003年第11期871-873,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的　观察钩端螺旋体DNA疫苗pcD flaB是否具有抗钩体交叉感染的保护作用。方法　将纯化后的钩体DNA疫苗pcD flaB 10 0 μg 2 0 0 μl肌肉注射豚鼠 ,共免疫 2次 ,间隔 7d ,初次免疫后的第 2 1天分别攻击感染赖型、临海型和波摩那型钩体 ,观察保护作用。结果　该疫苗对 3型钩体的保护率分别是 88.89%、10 0 %和 10 0 % ,总保护率为 96 .3% ,与对照组差异具有显著性。结论　DNA疫苗pcD flaB在豚鼠体内不但具有抗同型钩体 (临海型 )感染的作用 ,也具有较好的交叉保护作用。Objective To observe the protective effects of the DNA vaccine pcD-flaB on guinea-pigs from the infection by pathogenic Leptospira . Methods DNA vaccine pcD-flaB was given to the healthy guinea pigs i.m. on day 0, and boost seven days later. The animals were then challenged with three serovars of pathogenic Leptospira — L.serovar lai, L.serovar linhai and L.serovar pomona respectively. Results DNA vaccine pcD-flaB protected guinea pigs from leptospiral infection and the protective rates against the three serovars were 8/9, 9/9 and 9/9 respectively. Conclusion DNA vaccine pcD-flaB provided protection not only from the same serovar of Leptospira infection, but also from the different serovars of Leptospira infection.

关 键 词：钩端螺旋体 DNA疫苗 pcD-flaB 豚鼠 体内 免疫保护性 

分 类 号：R512.62[医药卫生—内科学]