苏云金芽孢杆菌抗蝗虫生物测定模式建立  

A Bioassay Model to Test Toxicity of Bacillus thuringiensis Against Locust

作　　者：彭可凡[1] 高梅影[1] 戴顺英[1] 安学芳[1] 朱幼玲[1] 蔡亚君[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国生物防治》2003年第4期189-192,共4页Chinese Journal of Biological Control

基　　金：国家自然科学基金资助课题(30170025)

摘　　要：在人工饲养条件下,研究了蝗虫种群的消长规律与环境因子的关系。结果表明,蝗虫每雌可产卵3～10块,每块达55.0粒,孵化率为76.32%,蝗蝻雌雄比为1.5～2∶1。建立了以蝗虫为试虫,苏云金芽孢杆菌的毒力生物测定模式。选择的最佳生测条件与蝗蝻的最适生长条件一致,温度30±1℃,湿度65%～75%,500～600lx光照14～15h。同时分析了蝗虫的饲料选择及各种环境因子对生测结果的影响。The relationships between Locusta migratoria manilensis and environmental factors were studied in the laboratory. The hatching rate, fecundity and sex ratio are 76.32%, 55.30 and 1.5-2∶1, respectively. A method of bioassay to estimate effect of Bacillus thuringiensis on L. migratoria manilensis was established. The effect of locust age, food and environmental factors e.g. temperature, relative humidity and photoperiod on the results of bioassay was also analyzed.

关 键 词：苏云金芽孢杆菌 蝗虫 模式 生物测定 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治] S433.2[农业科学—植物保护]