棉铃虫中肠氨基氨肽酶N基因的克隆及序列分析  

Cloning and Sequencing of a Gene Encoding Aminopeptidase N in the Mid-gut of Helicoverpa armigera (Hübner)

作　　者：王桂荣[1] 梁革梅[1] 吴孔明[1] 郭予元[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094

出　　处：《中国农业科学》2003年第11期1293-1300,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 0 3 0 0 2 3 3 );国家科技部 973资助项目 (G2 0 0 0 0 162 0 8)

摘　　要：通过简并引物PCR结合RACE技术 ,从棉铃虫中肠中克隆了 1个氨基氨肽酶N基因 ,该基因全长 30 4 3bp ,阅读框包括 2 85 6bp ,编码 95 1个氨基酸。预测蛋白分子量为 10 8.3kD ,等电点为 5 .2 9。序列中具有谷氨酸锌化氨肽酶的保守结构GAMEN以及锌结合位点HEXXHX18E。N 末端 2 0个氨基酸具有信号肽的特征 ,C 末端 2 5个氨基酸具有明显的亲脂性 ,并且具有跨膜特征。该基因已经在GenBank中登记 ,序列号为 :AY1810 2 6。A cDNA encoding aminopeptidase N was cloned by degenerated PCR combined with RACE technique. The full-length of APN-Harm was 3 043bp. Open reading frame is 2 856 bp in length, encoding 951 amino acid residues. Its predicted molecular weight and isoelectric point were 108.3 kD and 5.29, respectively. This deduced amino acid sequence shared some common structural features with aminopeptidase N from several moth species, including the consensus zinc-binding motif HEXXHX 18E and the GAMEN motif common to gluzincin aminopeptidases. The first 20 amino acid residues at C-end is hydrophobic transmembrane helix. The sequence of APN-Harm was deposited in GenBank and the accession number is AY181026.

关 键 词：棉铃虫 中肠 氨基氨肽酶 N基因 基因克隆 序列分析 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]