氰戊菊酯抗性和敏感品系棉蚜部分钠离子通道基因的克隆  

Cloning of Partial Sodium Channel Gene From Strains of Fenvalerate-resistant and Susceptible Cotton Aphid(Aphis gossypii Glover)

作　　者：孙鲁娟[1] 高希武[1] 郑炳宗[1] 

机构地区：[1]中国农业大学昆虫系,北京100094

出　　处：《中国农业科学》2003年第11期1301-1305,共5页Scientia Agricultura Sinica

基　　金：国家重大基础研究"973"资助项目 (G2 0 0 0 0 162 0 7);"863"害虫抗药性基因快速检测资助项目

摘　　要：在室内选育出了对氰戊菊酯抗性达 199.5 4倍的棉蚜品系。设计了 3条简并引物进行嵌套式PCR扩增 ,得到了氰戊菊酯敏感品系和抗性品系棉蚜钠离子通道基因IIS4 IIS6区的基因片段。序列分析表明 ,氰戊菊酯抗性品系棉蚜没有发生kdr或super kdr突变 ,但是与敏感品系相比 ,抗性品系钠离子通道基因IIS4 IIS6区有 2个核苷酸发生了突变 (T2A和A34T) ,并由此导致了 1个氨基酸的突变 (M12L) 。The strain of fenvalerate-resistant cotton aphid was selected using fenvalerate insecticide in the laboratory. The resistance factor of the strain was 199.54. Three degenerate primers were designed and used to perform PCR amplification. A cDNA encoding partial sodium ion channel gene was cloned from the fenvalerate-susceptible and -resistant strains. There were two nucleotide acid differences between fenvalerate-resistant strain and -susceptible strain, resulting in an amino acid mutation (Met→Leu). It is predicted that the mutation is related to the fenvalerate resistance of cotton aphid.

关 键 词：氰戊菊酯 抗性 敏感品系 棉蚜 钠离子通道 基因吉隆 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]