小金海棠中抗缺铁相关基因的杂交分析  

Blotting Analysis of Genes Relate to Resistance to Iron Stress of Malus xiaojinensis Cheng et Jiang

作　　者：戚金亮[1] 卫功宏[2] 黄勤妮[2] 印莉萍[2] 韩振海[1] 

机构地区：[1]中国农业大学农学与生物技术学院果树分子生物学实验室,北京100094 [2]首都师范大学生物系遗传与生物工程重点实验室,北京100037

出　　处：《中国农业科学》2003年第11期1330-1332,共3页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 0 170 5 5 2 );教育部"高等院校青年教师科教奖励"基金资助项目;北京市自然科学基金重点实验室"果树逆境生理研究室"资助项目

摘　　要：以高效抗缺铁胁迫基因型苹果种小金海棠 (MalusxiaojinensisChengetJiang)为试材 ,以异源的玉米Fe(II) 转运蛋白基因 (irt)和小麦Nramp基因为探针进行了杂交分析。Southern杂交结果表明 ,小金海棠基因组中该两家族基因均存在。Northern杂交结果表明 ,在根中 ,irt的转录受缺铁胁迫诱导 ,并随缺铁胁迫处理天数的增加而加强 ;在根和叶中 。Using Fe(II)-transporter gene (irt)of maize and Nramp gene of wheat as probes, these two genes were investigated in Malus xiaojinensis Cheng et Jiang by blotting hybridization technique. Southern blot suggested that the genome of Malus xiaojinensis Cheng et Jiang had these two family genes. Northern blot suggested that the transcription of Fe(II)-transporter gene was induced and strengthened by iron stress in roots; The transcription of Nramp gene was induced in both roots and leaves and strengthened in leaves by iron stress. irt and Nramp genes of Malus xiaojinensis Cheng et Jiang may play a crucial role in resistance to iron stress.

关 键 词：小金海棠 抗缺铁基因 杂交分析 转录表达 胁迫机理 

分 类 号：S661[农业科学—果树学]