细小病毒B19诊断芯片探针的制备  

Preparation of human parvovirus B19 microarray probes

作　　者：吕梁[1] 马文丽[1] 孙朝晖[2] 马晓冬[1] 郑文岭[2] 

机构地区：[1]第一军医大学分子生物学研究所,广州510515 [2]广州军区广州总医院肿瘤分子生物学研究所,广州510010

出　　处：《广东医学》2003年第12期1284-1285,共2页Guangdong Medical Journal

基　　金：广州市重点科技攻关项目 (编号 :2 0 0 1 -Z - 0 0 5- 0 1 )

摘　　要：目的　制备细小病毒B19诊断芯片探针。方法　利用PrimerPremier 5 0软件针对细小病毒B19基因保守区域设计PCR引物 ,纯化扩增产物 ,克隆鉴定。结果　序列分析表明 ,扩增片段均为细小病毒B19特异基因。结论　利用PCR扩增产物可制备细小病毒B19诊断芯片探针。Objective To prepare human parvovirus B19 probes for microarray detection. Methods Specific primers of PCR were designed with the Primer Premier 5.0 program according to the conserved regions of human parvovirus B19. Results Sequences were aligned, and the results showed that the products of PCR amplification were the specific gene fragments of human parvovirus B19. Conclusion It is a simple and effective method to prepare probes with PCR amplification of the conserved and specific human parvovirus B19 genes.

关 键 词：细小病毒B19 诊断芯片探针 制备 聚合酶链反应 

分 类 号：R392-33[医药卫生—免疫学]