从诸葛菜cDNA文库中筛选过氧化物酶基因  

To Construct the cDNA Library of Orychophragmus violaceus and Screen the cDNA Sequence of Peroxidase Gene(OvRCI)

作　　者：杜世章[1] 王劲[1] 李旭锋[2] 

机构地区：[1]绵阳师范学院生命科学技术系,四川绵阳621000 [2]四川大学生命科学学院,成都61064

出　　处：《四川大学学报（自然科学版）》2003年第6期1187-1190,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：绵阳师范学院科研基金

摘　　要：在200mmol/LNaCl诱导下构建诸葛菜苗期的cDNA文库,并从cDNA文库中筛选到一个新的过氧化物酶基因OvRCI,该基因cDNA全长1220bp,包含了一个1128bp的开放阅读框.通过同源分析比较及RT PCR实验等对该基因进行了分析.结果显示该基因是一个诱导型表达的基因.New peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of Orychophragmus violaceus peroxidase gene(OvRCI, GenBank Acc. No.bankit 569146) was 1220bp and contained a 1128bp open reading frame encoding a protein of 376 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. RT-PCR analysis revealed that OvRCI was salt-inducible and its transcript level was most abundant after 24 h treatment with 200 mmol/L sodium chloride. This studies suggested that OvRCIwas a new member of the family of recently cloned peroxidase genes.

关 键 词：诸葛菜 CDNA OvRCI RT-PCR 

分 类 号：Q785[生物学—分子生物学]