MGP(T-138C)基因多态性与儿童血铅水平关系的研究  

Study on association of polymorphism of MGP(T-138C) gene and blood lead level in children

作　　者：张文众[1] 冷曙光[1] 戴宇飞[1] 王雅文[1] 牛勇[1] 阎慧芳[1] 吴宜群[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2003年第6期514-515,共2页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .39770 6 40 )

摘　　要：为探讨基质γ 羧基谷氨酸蛋白 (MGP)基因多态性和血铅水平的关系 ,寻找与儿童铅中毒易感性有关的基因 ,测定了 3 55名 6～ 12岁的中国汉族儿童的血铅水平。采用多聚酶链反应 -限制性片段长度多态性法 (PCR RFLP)分析MGP基因T 13 8C位点的多态性。结果表明 ,在铅污染地区TT基因型儿童的血铅水平显著高于CC基因型儿童 (468 2 4μg L .vs .43 0 3 1μg L ,P <0 0 5)。结果显示MGP基因启动子区T 13In order to explore the relationship between polymorph ism of matrix γ-carboxyglutamic acid protein (MGP) gene and blood lead level i n children and to screen the gene which associated with lead poisoning, three hu ndreds and fifty-five children aged at 6 to 12 years old living in lead polluti on area were recruited in this study. The blood lead level were measured. The T-138 C polymorphism of MGP gene was analyzed with the method of polymerase chain reac tion-restriction fragment length polymorphism(PCR-RFLP). The blood lead level in children with wild type homozygotes(TT) was higher than those with mutated ho mozygotes(CC) (468 24μg/L. vs .430 31μg/L, P <0 05). The results indica ted that the T-138C polymorphism of MGP gene in promoter region influenced blood lead level in children lived in lead pollution area.

关 键 词：MGP基因多态性 血铅水平 遗传易感性 儿童 基质γ-羧基谷氨酸蛋白 铅中毒 

分 类 号：R595.2[医药卫生—内科学] R179[医药卫生—临床医学]