细颗粒物污染及对炎性因子IL-6表达的影响  

Fine particles pollution and its impact on the expression of inflammation cyto kine IL-6

作　　者：张文丽[1] 崔九思[1] 戚其平[1] 徐东群[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《卫生研究》2003年第6期548-552,共5页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .2 0 0 770 33)

摘　　要：为比较细颗粒物污染水平及研究其炎性损伤毒性 ,选取我国两个不同污染特征的城市太原与北京(燃煤污染 ,燃煤与汽车尾气混合污染 ) ,采用分级采样器收集两城市空气中细颗粒物样品 ,用重量法分析细颗粒物质量浓度 ,细颗粒物上的B(a)P用甲醇超声提取 ,Pb用硝酸和过氧化氢提取 ,比较分析了两城市细颗粒物污染水平 ;同时用ELISA及RT PCR法 ,以人肺泡上皮细胞 (A5 49)作为靶细胞 ,测定了细颗粒物对上皮细胞产生的炎性因子IL 6表达的影响。结果显示 ,以美国EPA大气环境质量PM2 5 标准为参考 ,PM2 5 太原冬季、北京冬季超标率、超标倍数分别为 10 0 % ,4 2 3 ;90 6% ,2 62。太原冬季、北京冬季空气中B(a)P浓度分别为58 6和 10 9ng m3 ,均超过我国标准。北京、太原空气颗粒物上Pb含量均未超过我国居住区大气Pb标准。细颗粒物能引起人肺上皮细胞产生炎性因子IL 6及其mRNA的表达增加 ,而且呈现剂量 -效应关系。本次研究地区空气细颗粒物污染比较严重 。To investigate fine particles (PM 2 5 )pollution and its imp act on the expression of inflammation cytokine IL-6. Two cities, namely Taiyuan and Beijing, were selected to represent two different types of air poll ution. Classification air sampler was used for PM 2 5 collection in the ai r, and weighting method was used to analyze PM 2 5 mass concentration. B (a) P in PM 2 5 was extracted by methyl alcohol using ultrasonic method and Pb in PM 2 5 w as diss olved in nitric acid and hydrogen peroxide. Human alveolar epithelial cell line (A 549 ) was used as target cell. Concentration of inflammation cytokine IL -6 was measured by an ELISA kit and, the mRNA expression of IL-6 was measured by RT-P CR while A 549 cells were incubated with PM 2 5 in different doses. Air Qu ality Standard for PM 2 5 of U S EPA was applied as the reference. The pe rcentag e and multiple of exceeding standard of PM 2 5 concentration of Taiyuan wi nter, B eijing winter and Beijing spring were 100%, 4 23; 90 6%, 2 62; 97 4%, 2 53 respe ctively. B(a)P in Taiyuan winter and Beijing winter were 5 86, 1 09 μg/100m 3) respectively that was over the standard Pb level of particle matter in two cit i es was under the Air Guality Standard of Residential Area in China. It was showe d an increase of protein concentration and mRNA expression of IL-6 in A 549 cultu red with PM 2 5 compared with untreated cells, presenting a dose-effect r elations hip. It is suggested thatPM 2 5 pollution was relatively heavy in the stud y areas and showed inflammation damage toxicity.

关 键 词：空气污染 细颗粒物 炎性损伤 IL-6 剂量-效应关系 基因表达 生物效应 

分 类 号：X513.032[环境科学与工程—环境工程]