枸杞多糖对小鼠睾丸细胞DNA损伤的保护作用  

Study on protective action of lycium barbarum polysaccharides on DNA imparments of testicle cells in mice

作　　者：黄晓兰[1] 杨明亮[1] 吴晓旻[1] 闫俊[1] 罗琼[1] 

机构地区：[1]武汉大学公共卫生学院,武汉430071

出　　处：《卫生研究》2003年第6期599-601,共3页Journal of Hygiene Research

基　　金：湖北省科技厅重点攻关项目 (No .2 0 0 1AA30 7B);达能营养中心膳食营养研究与宣教基金(No .2 0 0 30 7)

摘　　要：目的 :研究枸杞多糖 (LBP)对过氧化氢 (H2 O2 )引起的睾丸细胞DNA氧化损伤的保护作用。方法 :睾丸细胞先用不同浓度LBP作用 1h ,再加入 10 0 μmol LH2 O2 共同培养 2 5min ,然后用单细胞凝胶电泳技术(SCGE)测定DNA链断裂情况 ,分析LBP的抗氧化损伤作用。结果 :H2 O2 作用一定时间后 ,可引起DNA链断裂。 50、10 0、2 0 0、40 0 μg mL的LBP预处理可使H2 O2 染毒细胞的拖尾百分率和尾长显著降低。结论 :LBP本身没有致氧化损伤作用 ,它能够清除自由基 。To investigate the protective effect of lyc ium barbarum polysaccharides (LBP) on DNA oxidative damage of testicle cells ind uced by hydrogen peroxide (H 2O 2). The single cell gel electrophores is(SCGE) was used to detect the breakage of DNA strand and analyze LBP protectio n against oxidation damage in testicle cells treated by different concentrations of LBP for 1hour firstly, and then cultured with 100μmol/L H 2O 2 for 25 min . The results showed that a certain moment action by H 2O 2 could induce the breakage of DNA strand. The pretreatment of LBP (50,100,200,400μg/ml) significantly decrease d the frequencies of cells with tail moment and the tail length of testicle cell s treated by H 2O 2.It is suggested that LBP itself could not cause the oxidat ion damag e, but it could clean out the free-radical and restrain the DNA damage of testi cle cells caused by the oxidative stress.

关 键 词：枸杞多糖 DNA氧化损伤 抗氧化 单细胞凝胶电泳 小鼠 保护作用 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学] Q539[医药卫生—中医学]