锥型与圆柱型液相色谱制备柱的比较研究  

Comparative Study between Conical Column and Cylindrical Column for Preparative Liquid Chromatography

作　　者：马继平[1] 陈令新[1] 谭峰[1] 关亚风[1] 

机构地区：[1]中国科学院大连化学物理研究所

出　　处：《分析化学》2003年第11期1317-1321,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 0 0 75 0 3 0 )

摘　　要：用可视化柱上图形分析对入口内径大于出口内径的锥型液相色谱制备柱的样品谱带流型进行了研究。在一定的实验条件下 ,锥型柱与圆柱型柱的流动相流型曲线相反。对与锥型柱有相同长度、相同容积的圆柱型色谱柱的柱效、样品容量及峰高的比较研究表明 :锥型柱优于圆柱型柱。锥型柱的样品容量约为圆柱型柱的 2倍 ,柱效比圆柱型的高 36 % ,色谱流出曲线峰值高于圆柱型柱 12 %The flow profiles of solute bands in the conical column with larger inside diameter of inlet than outlet and cylindrical column were evaluated. by an on-column visualization method. In conical column, the band migrated slower in the central region than that in the wall region, resulting in a reverse parabolic flow profile contrast to that in cylindrical column. Compared with the cylindrical column with the same volume-and the same length, the conical column had double sample capacity, 36% higher column efficiency, and 12% higher peak height.

关 键 词：锥型柱 谱带流型 制备 高效液相色谱 可视化柱上检测法 

分 类 号：O657.7[理学—分析化学]