胶束增敏-同步荧光光谱法直接测定血浆中的依诺沙星  

Direct Determination of Enoxacin in Human Plasma by Micellar Enhancing Effect-Synchronous Fluorimetry

作　　者：杜黎明[1] 曹玺珉[1] 吴红艳[1] 

机构地区：[1]山西师范大学分析测试中心,临汾041004

出　　处：《分析化学》2003年第11期1330-1332,共3页Chinese Journal of Analytical Chemistry

基　　金：山西省自然科学基金资助项目 (No .2 0 0 110 16)

摘　　要：研究了胶束体系中依诺沙星的荧光性质 ,发现在pH 7.5的BR缓冲溶液中 ,十二烷基硫酸钠对依诺沙星有显著的增敏作用 ,据此提出了直接测定血浆中依诺沙星含量的等波长差同步荧光光谱法。其检出限为0 .10mg/L。经样品测定 ,回收率为 96 .9%～ 99.5 % ;相对标准偏差为 1.2 %～ 2 .3%。The fluorescence characteristics of enoxacin in micellar systems were studied in details. It was found that sodium dodecyl, sulfate could greatly enhance the fluorescence signal of enoxacin in. pH 7.5 Britton-robinson buffer solution. A new sensitive method using the synchronous, fluorimetry was developed for the direct determination of enoxacin in human plasma. The linear range is 0.10 similar to 3.5 mg/L, with a detection limit of 0.10 mg/L and the recovery of the method is within the range of 96.9% similar to 99.5% with a relative standard deviation below 2.3%.

关 键 词：胶束增敏 荧光光谱法 直接测定 血浆 依诺沙星 抗生素 血药浓度 

分 类 号：O657.3[理学—分析化学]