邻苯二酚紫-溴化十六烷基三甲铵-核酸共振光散射体系的研究及其分析应用  

Resonance Light Scattering of Catechol Violet-Cetyltrimethylammonium Bromide-Nucleic Acid System and Its Analytical Application

作　　者：陈艳晶[1] 杨景和[2] 吴霞[2] 曹伟[1] 庄海燕[1] 

机构地区：[1]济南大学化学与化工学院,济南250002 [2]山东大学化学与化工学院,济南250100

出　　处：《分析化学》2003年第11期1352-1355,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 9875 0 15 )

摘　　要：研究了核酸与阴离子染料邻苯二酚紫 (PV)及阳离子表面活性剂溴化十六烷基三甲铵 (CTMAB)体系的共振光散射光谱特性。在pH 2 .35的柠檬酸介质中 ,核酸 (yRNA或fsDNA)与阳离子表面活性剂CTMAB对邻苯二酚紫的共振光散射光谱有协同增强作用 ,产生最大散射波长为 4 0 0nm的共振光散射光信号。在最佳实验条件下 ,共振光散射测定yRNA和fsDNA的线性范围皆为 0 .0 2～ 0 .75mg/L ,检出限分别为 1.4和 8.7μg/L。This paper reports the spectral characteristic of resonance light scattering (RLS) system of catechol violet (PV)-cetyltrimethylammonium bromide (CTMAB)-nucleic acid. At pH 2.35, nucleic acid and CTMAB can cooperatively enhance the RLS intensity of PV and produce RLS with the maximum scattering peak located at 400 run. The enhanced RLS intensity is proportional to the nucleic acid concentration. Based on this phenomenon, ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) can be determined. The linear ranges of yRNA and fsDNA are both 20similar to750 mug/L, the limits of detection are 1.4 mug/L yRNA and 8.7 mug/L fsDNA. A new method for determination of nucleic acid was established.

关 键 词：邻苯二酚紫 溴化十六烷基三甲铵 核酸 共振光散射 测定方法 

分 类 号：O657.2[理学—分析化学]