HPLC法测定胆道感染患者胆汁中头孢曲松浓度  

Determination of the concentration of ceftriaxone in bile of biliary tract infection patients with HPLC

作　　者：韩丽萍[1] 张延军[1] 孙桂华[1] 骆科[1] 

机构地区：[1]广州军区广州总医院药学部,广东广州510010

出　　处：《广东药学院学报》2003年第4期316-318,共3页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的 :建立胆汁中头孢曲松浓度的测定方法 ,用于临床个体化给药监测。方法 :C1 8( 2 0 0mm× 4.6mm ,5 μm)色谱柱 ,流动相为 ψ[甲醇 ∶水 ( 0 .0 15mol/L溴化四丁基铵 ) ] =40∶60 ,流速 :1.0mL/min ,检测波长 2 5 4nm。结果 :日内和日间RSD均小于5 %。 8名患者静脉滴注头孢曲松 1g ,胆汁中药物峰浓度为 14 .3 7～ 2 90 7μg/mL ,8h内胆汁排泄率 0 .2 8%～ 3 7.3 %,个体差异较大。结论 :方法简便、快速 ,可用于胆汁样品的CTRX浓度测定。胆汁中CTRX浓度取决于患者胆汁分泌情况 ,个体差异较大 。Objective: To establish a method for the determination of ceftriaxone concentration in bile so as to monitor the clinical drug use. Methods: Spherisorb C 18 (4.6 mm×200 mm) column was used. Methanol ∶ water(0.015 mol/L Tetrabutyl Ammonium Bromide)(40 ∶ 60)was used as the mobile phase .The detection wavelength was 254 nm. Results: The level of RSD within day and between day was ≤5%. 1.0 g ceftriaxone was given to each of 8 patients by intravenous drip and it reached C max = 19.05～2907μg/mL,bile excrete rate of CTRX was 0.28%～37.3% in 8 h. The individual diversity was prominent. Conclusion: The method is simple and convenient,and applicable to determine drug concentration in bile.

关 键 词：头孢曲松 HPLC 胆道感染 个体化给药 

分 类 号：R969.1[医药卫生—药理学]