成年猫备用背根节内神经营养素-3对节内神经元的神经营养作用  

The Neurotrophic Effect of Endogenous NT-3 from Adult Cat Spared Dorsal Root Ganglion on Ganglionic Neurons

作　　者：章为[1] 周雪[1] 王廷华[2] 王特为[2] 刘苏[2] 陈思秀[1] 欧可群[1] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎与神经生物学教研室,成都610041 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2004年第1期25-28,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 980 0 191);四川省科委基金资助

摘　　要：目的　探讨成年猫部分去传入后备用背根节内神经营养素 - 3对节内神经元的神经营养作用。方法　切断 L1 、L3、L5和 L7背根 ,术后第 7d摘取 L2 、L4 和 L6 背根节做分离细胞培养 ,对正常背根节、备用背根节和备用背根节加抗体封闭 3个实验组中神经元存活、突起生长等情况进行观察比较。结果　备用背根节组存活细胞和细胞微团数目以及神经突起长度均明显高于正常组和抗体封闭组。结论　备用背根节内神经营养素 - 3对节内神经元有维持存活、促进突起生长的作用。Objective To investigate the neurotrophic effect of endogenous NT-3 from adult cat dorsal root ganglion (DRG) on ganglionic neurons. Methods Rhizotomy of bilateral L 1, L 3, L 5 and L 7 dorsal roots of cats was performed, leaving L 2, L 4 and L 6 DRG as spared DRGs. The separate neurons of normal (control) DRG, spared DRG and anti-NT-3 antibody blocking DRG were cultured in vitro respectively. The number of survival neurons and the length of neurites were measured and used for comparison in the control, spared DRG, and block groups. Results There were survival neurons and cell clusters in every group. The number of survival neurons and cell clusters of spared DRG group were much larger than those of the control and block groups. The neurite length of neurons, the neurite number and the length of cell clusters of spared DRG group were much greater than those of control and block groups. Conclusion Endogenous NT-3 from spared DRG may act on ganglionic neurons to maintain survival of neuron and stimulate growth of neurite.

关 键 词：备用背根节 神经营养素-3 培养 成年猫 

分 类 号：R33[医药卫生—人体生理学]