雌激素对低切应力作用下内皮细胞基因表达谱的影响  

Effects of 17 β-Estradiol on the Gene Expression of Shear Stressed Endothelial Cells

作　　者：张文胜[1] 成敏[2] 陈槐卿[2] 吴文超[2] 黄华[2] 

机构地区：[1]四川大学华西医院麻醉科,成都610041 [2]四川大学华西基础医学与法医学院生物医学工程研究室

出　　处：《四川大学学报（医学版）》2004年第1期5-7,24,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金重点项目 (批准号 3 983 0 110 ); CMB资助

摘　　要：目的　研究雌激素对低切应力作用下人脐静脉内皮细胞基因表达谱的影响 ,探讨雌激素对内皮细胞保护作用的机制。方法　用含 4 0 96点的 c DNA芯片高通量、大范围、高效率地检测经 17β-雌二醇 (10 - 7mol/L)孵育 4 8h后再行低切应力 (4 .2 0 dyne/cm2 ,2 h)作用后的人脐静脉内皮细胞的基因表达谱情况。结果　先用雌激素孵育再行低切应力处理的内皮细胞与未经任何处理的对照组比较 ,共有 384条基因出现差异表达 ,约占芯片基因总数的 0 .0 94 %。其中 2 2 6条基因表达增加 ,15 8条基因表达下降。在表达上调的基因中其转录产物涉及 PGI2 合成、单核 /巨噬细胞的吞噬作用等 ;在表达下调的基因中其转录产物涉及纤维蛋白原的降解、原癌基因产物的生成等。结论　生理浓度的雌激素对损伤内皮细胞有保护作用 ,其机制涉及到多种基因的调节。Objective To determine the protective effect of 17 β-estradiol on endothelial cells under shear stress. Methods cDNA microarray technology was adopted in the analysis of gene expression of cultured human umbilical vein endothelial cells (HUVECs) that had been incubated by 17 β-estradiol for 48 h and then exposed to low fluid shear stress (4.20 dyne/cm 2) for 2 h.Normal static cultured HUVECs were selected as control. Total RNA from normal static cultured HUVECs was labeled by Cy3-dCTP, and the total RNA of HUVECs incubated by 17 β-estradiol (10 -7 mol/L) and then exposed to low shear stress was labeled by Cy5-dCTP. The expression ratios reported are the average from the two separate experiments. Results A total of 384 genes (approximately 0.094%) exhibited differential expression. 226 genes were up-regulated and 158 genes were down-regulated. The transcription protein of up-regulated genes related to synthesis of PGI 2, macrophage phagocytosis, and so on. The transcription protein of down-regulated genes related to degradation of fibrinogen, and to production of protooncogene. Conclusion Estrin at the physiological concentration could protect impaired endothelial cells. Its protective effect may be related with some genes.

关 键 词：基因芯片 切应力 内皮细胞 17Β-雌二醇 

分 类 号：R541.4[医药卫生—心血管疾病]