前β_1-HDL对细胞胆固醇流出的影响  

Effect of Purified Pre-β1 HDL on Efflux of 3H-cholesterol Loaded in Human Smooth Muscle Cells

作　　者：吴新伟[1] 杨鲁川[1] 徐燕华[1] 傅强[1] 傅明德[1] 

机构地区：[1]四川大学华西基础医学与法医学院载脂蛋白研究室,成都610041

出　　处：《四川大学学报（医学版）》2004年第1期18-20,共3页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会 ( CMB;No.82 -4 12 )资助

摘　　要：目的　比较不同高密度脂蛋白 (HDL)亚类对细胞胆固醇移出的影响。方法　利用 3H-胆固醇负载的人血管平滑肌细胞 (SMC)为材料 ,通过细胞胆固醇流出时间与清除率的双倒数作图求出最大清除率 (Emax)及移出时间常数 (Ke) ,以此比较不同 HDL 亚类促使细胞胆固醇移出的特性和强度。结果　前 β1 - HDL 对 SMC3H-胆固醇的 Emax高于 α- HDL 及不含 apo E的 HDL3(P<0 .0 1) ;而 Ke又小于后两者 (P<0 .0 1)。结论　前 β1 - HDL 在清除SMC3H -胆固醇的能力 (Emax)及效率 (Ke)上均明显高于α- HDL及不含 apo E的 HDL3,是比α- HDL更为有效的细胞移出胆固醇的接受体。Objective To compare the effects of different HDL subclasses on the efflux of 3H-cholesterol loaded human smooth muscle cells (SMCS).Methods 3H-cholesterol loaded human SMCS were incubated with purified pre-β 1 HDL, α-HDL and apoE-deficient HDL 3 respectively. The cholesterol effluxing capacity and incubating time of different HDL subclasses were detected, and were analysed by double-reciprocal mapping. Results It was found that the value of E max of pre-β 1 HDL is higher than that of α-HDL and apoE-deficient HDL 3(P<0.01), and the value of Ke of pre-β 1 HDL is lower than that of α-HDL and apoE-deficient HDL 3( P<0.01). Conclusion The results showed the effluxing capacity and efficiency of pre-β 1 HDL were greater than those of α-HDL and apoE-deficient HDL 3, thus suggesting that pre-β 1 HDL is a more efficient acceptor of cell-derived 3H-cholesterol.

关 键 词：前β1-HDL 血管平滑肌细胞 ^3H-胆固醇 

分 类 号：R543.5[医药卫生—心血管疾病]