测定血小板衍生生长因子活性方法的改进  

An Improved Method of Determining PDGF Activity

作　　者：王湛[1] 李充璧[1] 周明海[1] 王利春[1] 阿丽娅[1] 

机构地区：[1]内蒙古大学生物工程中心,呼和浩特010021

出　　处：《内蒙古大学学报（自然科学版）》2004年第1期37-39,共3页Journal of Inner Mongolia University：Natural Science Edition

摘　　要：以BALB/C小鼠胚胎成纤维细胞(BALB/C3T3)作为靶细胞,经20mmol/L醋酸钠平衡后的CM-Sepharose(CMS)吸附过的胎牛血清作为基础培养血清,应用[3-(4,5-dimethylthi-azolyl)-2,5-diphenyltetrazoliumbromide,MTT]方法测定血小板源生长因子(platelet-derivedgrowthfactor,PDGF)诱导细胞增殖的生物活性.结果表明,该方法具有良好的量效关系,且精确度、重现性均较高.The activity of platelet-derived growth factor( PDGF)was determined by the method of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide(MTT).BALB/C embryo fibroblast 3T3 cells were taken as the target cells.The cow foetus serum (CFS) absorbed by carboxymethylcellulose-sephose (CMS) was taken as a basic cultural serum.The results indicated that this method was more accurate and repeatable,and had a good relationship between the effects and quantities

关 键 词：CM—Sepharose BALB/C 3T3细胞 血小板源生长因子(PDGF) 

分 类 号：Q26[生物学—细胞生物学]