IL-13基因多态性与血清IL-13及嗜酸细胞阳离子蛋白水平的关系  

The relationship between IL-13 gene polymorphism and the levels of serum IL-13 and serum eosinophil cation protein in asthmatic children

作　　者：孙海平[1] 陈吉庆[2] 郭锡熔[2] 陈荣华[2] 

机构地区：[1]江苏省淮安市妇幼保健院,223002 [2]南京医科大学儿科系

出　　处：《中华医学遗传学杂志》2003年第6期547-548,共2页Chinese Journal of Medical Genetics

摘　　要：目的　探讨 IL- 13基因多态性与血清 IL- 13及嗜酸细胞阳离子蛋白 (eosinophil cationprotein,ECP)水平的关系及 IL- 13基因多态性在儿童哮喘发病机理中可能的作用。方法　应用限制性内切酶片段长度多态位点法检测 96例哮喘患儿及 5 3名正常对照组儿童 IL - 13内含子 3+192 3位点 C/ T基因多态性 ,并应用 EL ISA法测定血清 IL- 13水平 ,运用荧光酶联免疫法测定血清 ECP水平。结果　哮喘组患儿 IL - 13内含子 3+192 3位点 TT、TC基因型频率分布高于正常对照组 (P<0 .0 5 ) ,且 TT、TC基因型患儿血清 IL- 13、ECP水平较 CC基因型明显升高 (P<0 .0 1)。结论　 IL- 13基因多态性与血清 IL- 13及 ECP水平关系密切 ,提示 IL - 13基因多态性在儿童哮喘发病机理中可能起重要的作用。Objective To explore the relationship between IL-3 gene polymorphism and the levels of serum IL-3 and eosinophil cation protein (ECP) for understanding the role of IL-3 gene polymorphism in the mechanism of childhood asthma. Methods The method of restriction fragment length polymorphism (RFLP) was adopted in detecting +1923 site polymorphism of IL-13 gene in intron 3 region, ELISA was employed in detecting the level of serum IL-13, and fluorescent enzyme-linked immunoassay was used to detect the level of serum ECP. Results The frequency distribution of TT,TC genotypes of IL-13 Intron 3+1923 site in asthmatic children was higher than that of CC genetype in normal control(P<0.05), and the levels of serum IL-13 and ECP of TT,TC genotypes were significantly higher than those of CC genotype respectively(P<0.01). Conclusion The close relationship of IL-3 gene polymorphism with the levels of serum IL-13 and ECP suggests that IL-3 gene polymorphism may play an important role in the mechanism of childhood asthma.

关 键 词：IL-3基因 基因多态性 血清 IL-13 嗜酸细胞阳离子蛋白 儿童 哮喘 

分 类 号：R725.6[医药卫生—儿科]