RP-HPLC法测定丁香酸的药物动力学与生物利用度  

Study on the Pharmacokinetics and Bioavailability of Syringic Acid in Rabbits

作　　者：刘云海[1] 郭咸希[1] 吕正松 谢委[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]湖北省恩施州中心医院,恩施450000

出　　处：《中药材》2003年第11期798-801,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金课题 (№ 3 9870 872 );卫生部科学研究基金课题 (№ 98 2 110 )

摘　　要：目的 :研究板蓝根抗内毒素活性成分丁香酸的体内单剂量静脉注射动力学及腹腔注射生物利用度。方法 :分别经兔耳静脉和腹腔注射给药后 ,在不同时间经耳缘静脉取血 ,用RP HPLC法测定血浆药物浓度 ,绘制药 时曲线 ,拟合房室模型 ,计算药物动力学参数 ,及腹腔注射给药的生物利用度。结果 :静脉给药后 ,丁香酸在兔体内的药物动力学过程符合二室模型 ,腹腔注射的生物利用度为 86 2 7%。结论 :这一结果对板蓝根抗内毒素新药的开发与临床应用有参考价值。Objective:To study the pharmacokinetics and bioavailability of syringic acid(SA) in rabbits by HPLC.Method:After the rabbits were injected SA injection via ear vein and peritoneal cavity,blood was collected also via ear vein at different time-point.Then the concentration of SA in blood was determined by HPLC and the concentration-time curve was mapped to simulate a compartment model.So the pharmacokinetic parameters were calculated out.Several days after this injection,the drug was administered to the rabbits via ip and using the same method,another concentration-time curve was obtained.By comparing the AUC of the two curves,bioavailabiity was obtained.Results:The concentration-time curve of SA in rabbits after iv fitted in a two-compartment open model and the absolute bioavailability was 86.27%.Conclusion:The results were valuable for the clinical application of new anti-endotoxic drug from Radix Isatidis.

关 键 词：RP-HPLC法 测定 丁香酸 药物动力学 生物利用度 板蓝根 抗内毒素活性成分 

分 类 号：R282.71[医药卫生—中药学]