Leptin长型受体与神经肽Y在猪前脑内共存  

Coexistence of leptin long form receptor and neuropeptide Y in the forebrain of pig

作　　者：樊明欣[1] 雷治海[1] 李庆梅[1] 王红星[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《南京农业大学学报》2003年第4期90-92,共3页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金资助项目 (3 9970 5 5 5 )

摘　　要：用原位杂交与免疫组织化学相结合的方法研究了 5头三元杂交仔猪前脑内leptin长型受体mRNA与神经肽Y(NPY)免疫反应阳性物质的共存关系。实验结果表明 ,leptin长型受体mRNA和NPY免疫反应阳性神经元分布于下丘脑漏斗核和前室周核、海马、杏仁核及大脑皮质。上述结构内一些表达leptin长型受体mRNA的神经元也含有NPY免疫反应阳性物质 ,即leptin长型受体与NPY共存于同一神经元内。提示leptin可能通过下丘脑漏斗核和室周核以及边缘系统的海马和杏仁核 ,甚至大脑皮质中表达leptin长型受体mRNA的NPY免疫反应阳性神经元调节动物的摄食、能量代谢、生长和繁殖等活动。Combination of in situ hybridization and immunohistochemistry methods was used to investigate coexistence of the long form leptin receptor(OB-Rb)mRNA and neuropeptide Y-like immunoreactivity(NPY-IR)in neurons of the pig forebrain.The results showed that both OB-Rb mRNA and NPY-IR containing neurons were seen in the infundibular nucleus and periventricular nucleus in the hypothalamus,hippocampal formation,amygdala and cortex of the pig forebrain,and that some of neurons expressing OB-Rb mRNA also contained NPY-IR.It meant coexistence of OB-Rb and neuropeptide Y in these neurons of the pig forebrain.The results suggested that leptin may exert control of food intake,energy metabolism,growth and reproduction through the NPY neurons expressing OB-Rb.

关 键 词：Leptin长型受体 神经肽Y 猪 前脑 共存关系 原位杂交 免疫组织化学 瘦素 蛋白质激素 

分 类 号：S828.1[农业科学—畜牧学] Q57[农业科学—畜牧兽医]