乌苏里藜芦碱抗血栓作用的研究(英文)  

Antithrombotic effects of Veratrum nigrum var.ussuriense alkaloids

作　　者：韩国柱[1] 李欣燕[1] 吕莉[1] 李卫平[1] 赵伟杰[2] 

机构地区：[1]大连医科大学药理教研室,辽宁大连116027 [2]大连理工大学化学制药系,辽宁大连116027

出　　处：《中草药》2003年第12期1107-1110,共4页Chinese Traditional and Herbal Drugs

基　　金：辽宁省教育委员会资助项目 (2 0 2 72 2 71)

摘　　要：目的　研究乌苏里藜芦生物碱 ( Vn A)对大鼠的抗血栓作用。方法　分别应用电刺激诱发大鼠颈总动脉血栓形成模型和瘀血诱发下腔静脉血栓形成模型评价 Vn A的抗动、静脉血栓形成作用。结果　大鼠 iv6种不同剂量的 Vn A ( 7.2～ 4 2 .9μg/ kg)导致血管阻塞时间 ( OT)显著延长 ,且具明显的剂量依赖性 ,Vn A 30μg/ kg产生的抗动脉血栓形成作用与赖氨匹林 18.0 mg/ kg的作用相当。单次 iv Vn A30 μg/ kg使 OT呈时间依赖性延长。Vn A抗动脉血栓形成作用起效迅速 ,持续至少 80 min,iv后 15 m in达效应峰值。iv Vn A( 15～ 4 5 μg/ kg)显著减少下腔静脉血栓干质量 ,并呈剂量依赖性。结论　 Vn A具有强大的抗动、静脉血栓形成作用 ,并具明显的剂量依赖性和时间依赖性。其抗血栓效能高 ,作用强度大 ,在大鼠的有效剂量低至μg/ kg水平。VnObject To investigate antithrombotic effects of Veratrum nigrum L.var ussuriense Nakai alkaloids ( Vn A) in rats. Methods The electrically induced rat carotid artery thrombosis model was used to examine the antiarterial thrombosis effect of Vn A using occlusion time( OT) of carotid artery as phar- macologic index;the stasis- induced inferior vena cava thrombosis model was used to evaluate antivenous thrombosis effectof Vn A in terms of thrombosis formation rate and decrease in thrombus dry weight.Re- sults The iv administration of Vn A( 7.2— 42 .9) μg/kg to rats resulted in a dose- dependent effect and significantprolongation in OT. Vn A at the dose of 30μg/kg produced an antiarterial thrombosis effecte- qual to thatof LAS( 1 8.0 mg/kg) .Also,a single bolus iv Vn A( 30 μg/kg) increased OT in a time- depen- dentmanner;the antiarterial thrombosis effectwas rapid in onsetand lasted atleast80 min,and peaked at 1 5 min postdosing.Vn A( 1 5— 45 μg/kg iv) decreased the thrombus dry weight significantly and dose- de- pendently. Conclusion Vn A has powerful inhibitory effects againstboth arterial and venous thrombosis in rats and acts in a dose- and time- dependentmanner.Its effective dose is as low asμg perkg body weightof rats.The finding of the Vn A antithrombotic effeets reveal a bright future of its R &D.

关 键 词：血栓 乌苏里藜芦 生物碱 藜芦 

分 类 号：R286.3[医药卫生—中药学]