广西苗族人群15个STR基因座的多态性调查  

Genetic studies of 15 STR loci in Guangxi Miao population

作　　者：刘超[1] 杨电[1] 刘长晖[1] 朱少建[2] 李发贵[2] 汪萍[2] 

机构地区：[1]广州市刑事科学技术研究所,广东广州510030 [2]广西壮族自治区公安厅刑科所,广西南宁530012

出　　处：《法医学杂志》2003年第4期204-206,共3页Journal of Forensic Medicine

基　　金：公安部科技攻关项目(20004421401)

摘　　要：目的调查广西苗族人群无关个体的15个STR基因座(D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法应用AmpFlSTRIdentifilerTM荧光标记复合扩增系统对274例广西苗族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI3100遗传分析仪对扩增产物进行检测,用GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果IdentifilerTM荧光标记系统的15个STR基因座在广西苗族人群的累积偶合率为5.04×10-17,累积非父排除率分别为0.9999993。结论该15个STR基因座可满足广西苗族人群法医学的个体识别及亲权鉴定的需要。Objective The study was carried out to investigate genetic polymorphism of15STR loci in Guangxi Miao population.Methords DNA samples from southern China274Miao unrelated individuals were screened by using AmpFlSTR Identifiler TM PCR Amplification Kit and3100Genetic Analyzer.Results These15loci meet the Hardy-Weinberg expectations.The matching probability of the15STR loci was5.04 × 10 -17 .and combined pa - ternity of exclution was0.9999993in Guangxi Miao population.Conclusion Our results showed Identifiler TM PCR Amplification systems of15STR loci were useful enough to forensic case work in Guangxi Miao population.

关 键 词：广西 苗族人群 STR基因座 基因多态性 法医学 基因分型 

分 类 号：Q347[生物学—遗传学]