组织工程化肌腱修复鸡趾屈肌腱缺损的生物力学特性研究  

Biomechanical Properties of Tissue-engineered Tendons after Repairing Digital Flexor Tendons in Chickens

作　　者：秦廷武[1] 张姝江[1] 杨志明[1] 李秀群[1] 

机构地区：[1]四川大学华西医院骨科修复重建外科研究室,成都610041

出　　处：《生物医学工程学杂志》2003年第4期601-604,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (3 0 0 70 199);四川省青年科技基金资助课题 (2 0 0 1-19-0 13 2 )

摘　　要：组织工程化肌腱植入体内修复的肌腱其抗拉强度达不到正常肌腱的数值。为探讨这一问题的原因 ,我们选择罗曼雏鸡足趾屈肌腱细胞与可降解聚羟基乙酸筛网体外复合培养构建组织工程化肌腱。用此工程化肌腱修复 2 0只罗曼鸡第二趾深屈肌腱 0 .5～ 0 .8cm缺损。术后第 2、4、6、8周取材 ,测定样品中材料的重量、羟脯氨酸含量及抗拉强度等力学特性指标。结果显示 ,植入 2、4、6、8周 ,支架材料重量下降很快 ,至第 8周基本降解 ;修复的肌腱中代表胶原合成总量的羟脯氨酸含量随时间增加 ,但变化不明显 ;修复的肌腱断裂能量和抗拉强度均随时间呈一先降低后逐渐增大的变化 ,抗拉强度在第 8周才达到正常肌腱的 2 3%。结果提示 ,植入的组织工程化肌腱在其材料迅速降解的同时 ,胶原生成量并不多 ,二者出现明显的不匹配 。Experiments have been performed to investigate why the biomechanical strength of repaired tendons is lower than that of the normal tendon when the engineered tendons are implanted in vivo to replace the tendon defects. We seeded the primary culture tendon cells derived from Roman chickens' digital flexor tendons on the degradable polyglycolic acid meshes to construct tissue engineered tendons. The flexor tendon defects (0.5 cm 0 8 cm) excised in second digit bilaterally in 20 Roman chickens, had been repaired with the constructed tissue engineered tendons. The samples of repaired tendons were collected at 2, 4, 6 and 8 weeks afteroperation. Tests for scaffold weight, hydroxyproline content, and mechanical strength of the samples were performed. We found that from 2 weeks to 8 weeks afteroperation, the weight of the scaffolds decreased significantly, almost disappearing at 8 weeks; the hydroxyproline content determining the total collagen content increased gradually without significance; mechanically, both energy at break and tensile strength showed a tendency of drastic decrease at first 4 weeks afteroperation and a gradual increase afterwards, but the tensile strength at 8 weeks afteroperation was only 23% of that of the normal tendon. We conclude that the lower biomechanical strength of repaired tendons is owing to the serious mismatch between scaffold degradation and collagen synthesis.

关 键 词：组织工程化肌腱 修复 鸡趾屈肌腱缺损 生物力学特性 抗拉强度 

分 类 号：R687.2[医药卫生—骨科学]