棉花基因枪茎尖转化研究  

Study on Transformation of Meri- stem via Gene-gun Bombardment

作　　者：刘传亮[1] 于娅[2] 王玉芬[1] 耿立召[1] 李付广[1] 

机构地区：[1]中国农业科学院棉花研究所农业部棉花遗传改良重点开放实验室,河南安阳455004 [2]河北农业大学,河北保定071000

出　　处：《棉花学报》2003年第6期361-366,共6页Cotton Science

基　　金：863计划及国家转基因植物研究专项资助(J2002-B-001)

摘　　要：对棉花茎尖轰击转化的多种因素进行了分析,建立了较为完善的基因枪茎尖转化体系,使抗性转化率达到4.7％～9.4％,转化周期缩短至2～3个月,改进了抗性筛选程序,建立了4次筛选法,获得91株抗生素抗性植株;同时对部分植株目的基因的表达量进行了初步的遗传分析。结果表明,茎尖的轰击状态与抗性筛选程序对转化周期与转化率具有决定性作用。An improved transformation system of meristem via gene-gun bombardment was established after studies on many influential factors. The transformation rate of anti-antibiotic reached 4. 7% -9. 4% and the transformation cycle decreased to 2-3 months; Four-times Selective Strategy was founded after improving selection of transformation plants; 91 anti-antibiotic transgenic plants were obtained, some of them had been carried out their Bt measurements in leaves and analyses of their genetic rules. All the results showed the state of the shoot-tips and the Selective Strategy acted vital roles on transformation rate and cycle.

关 键 词：棉花 茎尖 基因枪 遗传转化 抗性 抗生素 植株 表达量 遗传分析 筛选程序 转化周期 转化率 

分 类 号：S562[农业科学—作物学]