反相高效液相色谱法测定尿中右美沙芬及去甲右美沙芬的含量与CYP2D6表型分析  

Determination of dextromethorphan and dextrophan in human urine of Han,Uigur and Kazak nationalities by RP-HPLC

作　　者：李国昌[1] 洪成林[2] 陈文[2] 陈勇[1] 

机构地区：[1]石河子大学医学院第一附属医院药剂科,新疆石河子832008 [2]石河子大学药学院,新疆石河子832002

出　　处：《中国药学杂志》2003年第11期862-864,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目 (3 9960 0 80 )

摘　　要：目的　建立RP HPLC测定不同种族人尿中右美沙芬及去甲右美沙芬含量的方法。方法　尿样经酶水解 ,酸、碱提取纯化后 ,2 0 μL直接进样于HPLC系统 ,经苯基柱分离后荧光 (激发波长 2 80nm ,发射波长 310nm)检测 ,流动相为乙腈 10mmol·L-1KH2 PO4,10mmol·L-11 己烷磺酸钠缓冲溶液 ( 5 1∶4 9,pH 4 .0 ) ,柱温为 2 5℃。 结果　样品中右美沙芬及去甲右美沙芬得到较好分离 ,线性范围右美沙芬在 0 .0 2～ 2 μg·mL-1,r =0 .9998,n =9,最低检测限为 0 .4ng·mL-1。去甲右美沙芬 0 .5～ 30μg·mL-1,r=0 .9985 ,n =7,最低检测限为 0 .8ng·mL-1。结论　该方法简便可靠 。OBJECTIVE: To extablish a reverse phase HPLC method for the determination of dextromethorphan and dextrophan in human urine in different races. METHODS: The urine samples were hydrolyzed by enzyme, distilled and purified by acid and alkali. Then 20 μL extracted sample was directly injected into the HPLC system with Phenyl colunm, and fluorescence detection (ER at 280 nm, EX at 310 nm). Acetonitrile-water containing 10 mmol&middotL-1 KH 2PO4 and 10 mmol&middotL-1 1-hexane sulfonate-Na (51:49, pH4.0) was used as the mobile phase. The column temperature was 25°C. RESULTS: The dextromethorphan and dextrophan were seperated satisfactorily. The linear ranges of dextromethorphan and dextrophan were 0.02∼2 μg&middotmL-1 r = 0.999 8, n, = 9) and 0. 5∼30 μg&middot mL-1,respectivly. The detection limits were 0.4 and 0.8 ngl&middotmL-1, respectivily. CONCLUSION: The method is simple and reliable. The results shows that RP-HPLC is a good method for measuring both dextromethorphan and dextrophan in human urine in different races and for studying the phenotype of the genetic polymorphisms.

关 键 词：反相高效液相色谱法 测定 尿 右美沙芬 去甲右美沙芬 含量 CYP2D6 表型 

分 类 号：R974.2[医药卫生—药品] R969.1[医药卫生—药学]