黄芪对低氧性肺动脉高压大鼠肺血管结构重建干预作用及机制的研究  

INFLUENCE OF RADIX ASTRAGALI ON STRUCTURAL REMODELING OF INTRA-ACINAR PULMONARY ARTERY IN RATS WITH HYPOXEMIC PULMONARY HYPERTENSION

作　　者：安昌善[1] 柳济成[1] 孙红花[1] 吴美玉[1] 

机构地区：[1]延边大学医学院附属医院呼吸内科,吉林省延吉市133000

出　　处：《中国心血管病研究》2003年第2期146-148,共3页Chinese Journal of Cardiovascular Research

基　　金：延边朝鲜族自治州科技局资助项目(9919-3)

摘　　要：目的 探讨黄芪注射液对低氧性肺动脉高压(HPH)大鼠肺腺泡内肺动脉结构重建及肺组织内皮素-1(ET-1)、一氧化氮(NO)的影响。方法雄性Wistar大鼠21只,每组7只,随机分为3组,正常对照组、低氧组、低氧+黄芪治疗组。低氧30d,分别用右心导管法测定肺动脉平均压(mPAP)、颈动脉平均压(mCAP),右心室肥大指数(RVHI),分光光度法测定肺组织中NO含量和放射免疫法测定肺组织中ET-1含量,计算MA、PMA、NMA比例及肺中、小肌型动脉相对中膜厚度(RMT)与相对中膜面积(RMA)。结果①低氧组mPAP、RVHI、ET-1含量较对照组明显升高(P<0.01或P<O.05),治疗组较低氧组明显减少(P<O.05);②低氧组NO含量较对照组降低(P<0.01),治疗组较低氧组升高(P<O.05);③低氧组肺小动脉肌化程度、RMT(％)、RMA(％)较对照组明显增多(P<O.01),治疗组较低氧组明显减少(P<O.01);④reCAP 3组之间比较差异无显著性(P>0.05)。结论慢性低氧可引起大鼠肺血管结构重建,导致mPAP上升、肺组织ET-1升高、NO下降,黄芪注射液可调节肺组织ET-1、NO含量,可部分逆转肺血管结构重建,降低肺动脉高压,对HPH有一定防治作用。Objective To investigate the effects of Radix Astragali on structural remodeling of intra -acinar pulmonary artery(IAPA) and levels of NO、ET- 1 in lung tissues of hypoxemic pulmonary hypertension rats. Methods 21 male Wistar rats were divided into three groups: normal control group (N); hypoxia group (H); hypoxia + Raidx Astragali group( T) . Rats in group T were treated with . Radix Astragali(12g/kg intra - peritoneal injection) . Levels of MPAP、mCAP、RVHI、NO、ET- 1 were measured. Degrees of IAPA and RMT(relative medial thickness)、 RMA( relative medial acreage)of intra- acinar pulmonary artery were measured. Results ①Levels of MPAP, RVHI, ET - 1 in group T were significantly lower than that in group H(P<0. 05). ②Level of NO in group T was significantly increased than that in group H(P<0.05).③ Levels of RMT、 RMA and rates of MA (muscular artery) 、 PAM( partal muscular artery) in group H were significantly higher than that in group C(P< 0. 01). Levels of RMT、RMA and the rates of MA、RMA in group T were significantly lower than that in group H(P<0. 01) .The rate of NMA(non muscular artery)in group T was significantly higher that in group H. ④There was no significantly difference in mCAP among three groups(P>0. 05) . Conclusion Chronic hypoxia may result in reconstruction in pulmonary vessels. Injection of Radix Astraglai may reverse the remodeling of Intra-acinar pulmonary artery partly and play a role in hypoxemic pulmonary hypertension.

关 键 词：黄芪注射液 低氧性肺动脉高压 大鼠 肺血管 HPH 肺组织 

分 类 号：R563[医药卫生—呼吸系统] R-332[医药卫生—内科学]