IL-1β增强主动脉平滑肌细胞内Ⅰ型IP_3受体表达  

Interleukin-1β enhanced type I inositol 1,4,5-triphosphate receptor expression in rat aortic arterioles smooth muscle cells

作　　者：王静艳[1] 吕飒[1] 刘沛[1] 

机构地区：[1]中国医科大学第二附属医院感染科,沈阳110004

出　　处：《中国免疫学杂志》2003年第12期821-824,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助 ( 3 0 170 849)

摘　　要：目的 :探讨白细胞介素 1(IL 1)对主动脉平滑肌细胞株 (A7r5细胞 )Ⅰ型IP3受体蛋白过度表达的影响。方法 :通过对主动脉平滑肌细胞培养 ,应用蛋白杂交技术、核酸杂交技术分别检测在IL 1β作用后 ,主动脉平滑肌细胞中Ⅰ型三磷酸肌醇 (IP3)受体蛋白和Ⅰ型IP3受体mRNA的表达及Ⅰ型IP3受体mRNA半衰期的测定 ,同时研究IL 1β对Ⅰ型IP3受体基因启动子的作用。结果 :IL 1β能增强主动脉平滑肌细胞内的Ⅰ型IP3受体蛋白的表达 ,两者呈剂量依赖关系 ;IL 1β也能使Ⅰ型IP3受体mRNA水平增高 ;通过对Ⅰ型IP3受体mRNA半衰期的测定明确IL 1β对Ⅰ型IP3受体mRNA影响不是抑制mRNA的降解 ,而是诱导其表达增加。IL 1β作用 4小时时 ,装有Ⅰ型IP3受体启动子的质粒上的报告基因表达增加 ,而 8小时时 ,报告基因的表达即降至IL 1β作用前的水平。 结论 :IL 1可能作用于Ⅰ型IP3受体mRNA的基因启动子 ,使Ⅰ型IP3受体mRNA合成增加 ,导致Ⅰ型IP3受体蛋白表达增加 ,由此构成了主动脉平滑肌细胞内储备Ca2 + 释放 ,引起动脉平滑肌细胞收缩、舒张功能异常的病理生理基础。Objective:In order to investigate interleukin-1 (IL-1) affects on type I inositol 1,4,5-triphosphate receptor (IP 3 R) expression in rat aortic arterioles smooth muscle cells (A7r5 cells).Methods:Aortic arterioles smooth muscle cells were isolated and cultured, and the protein and mRNA of type I IP 3 R and report gene in aortic arterioles smooth muscle cells treated with IL-1 were detected by Western blot, Northern blot and Dual-luciferase reporter assay system. Results:IL-1β enhanced the expression of type I IP 3 R protein and type I IP 3 R mRNA in aortic arterioles smooth muscle cells; IL-1β did not influence the degeneration of type I IP 3R mRNA in aortic arterioles smooth muscle cells after treated by IL-1β. The activity of the type I IP 3 R promoter was increased after treatment with IL-1β for 4h, then dropped to 0h level at 8h.Conclusion:IL-1 maybe act on the promoter of type I IP 3 R mRNA in aortic arterioles smooth muscle cells and result in the expression of type I IP 3 R protein that stimulates releasing of intracellular Ca 2+ in aortic arterioles smooth muscle cells, inducing abnormal vesocostriction function of aortic arterioles smooth muscle and ensuing the septic shock.

关 键 词：白细胞介素1 三磷酸肌醇 受体 主动脉平滑肌细胞 

分 类 号：R392.12[医药卫生—免疫学]