大豆DNA导入引起大麦籽粒蛋白含量及氨基酸含量变异的遗传稳定性分析  

Analyses on the Hereditary Stability of Protein Content and Quality Variation In Barley Seeds By Introducing Exogenous Soybean DNA

作　　者：毛万霞[1] 许燕[1] 张国荣[2] 李建粤[1] 吕英海[1] 陆雪琴[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《种子》2003年第6期6-9,共4页Seed

基　　金：上海市农委科技兴农重点攻关项目;编号为农科攻字(98)第01-3号

摘　　要：采用花粉管通道法和两种不同压力基因枪法将大豆总DNA导入大麦,提高了大麦后代籽粒的蛋白质含量和必需氨基酸含量。四代大麦籽粒蛋白质含量追踪分析及第四代籽粒氨基酸含量分析结果表明,部分大麦籽粒的高蛋白含量及质量变异性状能够稳定遗传。本试验还分析比较了两种DNA导入法,采用基因枪法比花粉管通道法在保持后代高蛋白遗传稳定性方面效果更好。在两种不同压力基因枪处理中,1300psi压力处理比1500psi压力处理更适合大豆总DNA的导入。在本试验中我们还获得了两个高蛋白性状较稳定的穗行,它们的蛋白质平均含量分别比对照提高20.67%和7.991%。The protein contents and essential amino acid contents were improved in barley seeds by introducing exogenous DNA from soybean into barley through the pollen-tube pathway after self-pollination and particle bombardment. The protein contents and amino acid contents in four generation seeds were analyzed. The results indicated that the variation of high protein content and quality in the seeds of some progenies into which soybean DNA was induced could be stably heredity. The effectiveness was compared between pollen-tube pathway and particle bombardment. It was best by the way to introduce exogenous DNA by 1 300psi particle bombardment in keeping high protein contents in offspring seeds. Two high protein content stains were obtained in this research. Their protein contents in the seeds respectively increased by 20. 67% and 17. 99% over the contrast.

关 键 词：大豆 DNA导入 大麦 籽粒蛋白 含量 氨基酸 

分 类 号：S512.3[农业科学—作物学]