西藏砂生槐生物碱抑菌抑瘤的实验研究  

A study on bacteriostasis and inhibiting tumour cell proliferative activity of alkaloids from Sophora moorcroftiana seed

作　　者：马兴铭[1] 李红玉[1] 尹少甫[2] 孟雪琴[1] 周攻克[1] 陆晓明 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]兰州医学院免疫学教研室,甘肃兰州730000 [3]兰州同健生物科技股份有限公司,甘肃兰州730000

出　　处：《兰州大学学报（自然科学版）》2003年第6期74-77,共4页Journal of Lanzhou University(Natural Sciences)

摘　　要：砂生槐是西藏南河谷地带分布极广的一种多年生豆科灌木 ,为青藏高原特有植物种 ,是一种极为宝贵的植物资源 .我们首次从砂生槐种子中提取生物碱 ,用 K-B法和最低抑菌浓度测定法进行体外抑菌活性研究 ,用 MTT法测定生物碱对肿瘤细胞增殖的抑制作用 .结果表明砂生槐种子总生物碱具有抑制金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌生长繁殖的活性 ,对 SPC-A-1和 GLC-82肺腺癌细胞的增殖有明显的抑制作用 ,且抑制作用随药物浓度的增加和作用时间的延长而增强 。Sophora moorcroftiana is a kind of perennial leguminous bush,which is widely distributed in the Yaluzangbu Valley of Southern Tibet.It is a kind of very valuable natural plant and grows only in the Tibetan altiplano.Alkaloids were extracted from Sophora moorcroftiana seed in this study.The bacteriostasis activities of alkaloid in vitro were tested with K-B method and minimal inhibitory concentrations(MIC).Inhibition proliferation activities on tumour cell of alkaloid were measured by MTT assay.The results show there is a dramatic bacteriostasis activity on Staphyloccus aureus,Escherichia coli and Patina aerugionsa.SPC-A-1 cell and GLC-82 cell proliferation effect were inhibited by alkaloids in vitro.The antitumour activity was gradually enhanced with a alkaloid variety of time and dose.The discovery provides an important basis for possible medical application.

关 键 词：砂生槐 生物碱 抑菌作用 抑瘤作用 MTT法 

分 类 号：R285.5[医药卫生—中药学]