小麦全蚀病菌胞外β-1,3-葡聚糖酶的产生和部分特性的研究  

PRODUCTION AND PROPERTIES OFβ-1, 3- GLUCANASE PRODUCED BY GAEUMANNOMYCES GRAMINIS VAR.TRITICI

作　　者：王旭丽[1] 黄丽丽[1] 康振生[1] 郭蔼光[2] 刘铜[1] 

机构地区：[1]西北农林科技大学植保学院 [2]西北农林科技大学生物技术中心,陕西杨凌712100

出　　处：《菌物系统》2003年第4期628-633,共6页Mycosystema

基　　金：国家自然科学基金资助项目(30270863);西北农林科技大学校内重点项目

摘　　要：本文就小麦全蚀病菌胞外-1,3-葡聚糖酶的产生和部分酶学特性进行了研究。结果表明,小麦全蚀病菌能够产生胞外-1,3-葡聚糖酶。在供试的三种培养基中,最佳产酶培养基为改进的MS培养基。当以改进的MS为基础培养基时,最佳碳源为麦麸皮;最佳氮源为牛肉浸膏;产酶的最适条件为培养基初始pH为6,培养温度为26℃,250ml三角瓶中装培养基量为50ml时接菌量为4块菌饼(直径5mm)。另外,对酶的部分性质的研究结果表明,酶最适作用温度和pH分别为60℃和7.0,在50℃以下以及pH 5.5～7.5范围内稳定。The effect of culture conditions onβ-1, 3- glucanase production of Gaeumannomyces graminis var. tritici and partial properties of crude enzyme were studied. The results showed that improved MS medium gave the higher yield of totalβ-1,3- glucanase than Czapek-Dox medium and 1/2 potato dextrose medium. The suitable carbon source forβ-1,3- glucanase production was wheat bran. The suitable nitrogen source was beef extract. Initial pH of 5.5, incubation period of 21 days at 26℃, Quantity of 4 fungal disk (each 5 mm in diameter) in 50ml liquid medium in 250ml flask were the optimal conditions for enzyme production. The optimal temperature and pH of the enzyme activity were 60℃and 7.0, respectively. The enzyme was stable at pH5.5-7.5 and temperature below 50℃.

关 键 词：禾顶囊壳小麦变种 改进MS培养基 培养条件 酶学性质 

分 类 号：S432[农业科学—植物病理学]