中药抑抗灵治疗抗精子抗体介导的免疫性不育的实验研究Ⅱ  

Chinese Traditional Medicine Yi Kang Ling to Treat Immunity Infertility Caused by Anti-sperm Antibody:the Experimental Research Ⅱ

作　　者：戴晓莉[1] 崔英霞[2] 王咏梅[2] 胡毓安[2] 徐建平[2] 姚兵[2] 李保仝[2] 

机构地区：[1]南京军区南京总医院药剂科,江苏南京210002 [2]南京军区南京总医院生殖遗传研究室,江苏南京210002

出　　处：《中华男科学杂志》2003年第9期713-715,共3页National Journal of Andrology

基　　金：江苏省高新技术创业服务中心基金项目 (0 3 0 62 8)

摘　　要：目的 :观察中药抑抗灵对抗精子抗体 (AsAb)介导的免疫性不育的疗效。　方法 :在建立AsAb介导的免疫性不育动物模型的基础上 ,用抑抗灵对新西兰兔进行治疗 ,治疗完成即AsAb阴转率达到预期疗效时 ,雌雄兔交配 ,观察孕育结果。　结果 :原免疫状态雄 (+)雌 (- )抑抗灵治疗的低、高剂量组不育率为 2 0 % ,中剂量组为 0 ,强地松对照组为 0 ,未治疗组为 6 0 % ;雄 (- )雌 (+)低剂量组不育率为 0 ,中剂量组不育率为 2 0 % ,高剂量组不育率为2 5 % ,强地松对照组为 2 5 % ,未治疗组不育率为 4 0 % ;雄 (+)雌 (+) 3个剂量组的不育率均为 2 0 % ,强地松对照组为 2 0 % ,未治疗组不育率为 6 0 % ;阴性对照组不育率为 2 0 %。原免疫状态为雄 (+)雌 (- )抑抗灵低剂量组、中剂量组、高剂量组新生兔体重分别为 (5 1.7± 8.8) g ,(5 0 .5± 6 .4 ) g ,(5 3.5± 7.2 ) g ;强地松对照组和未治疗组新生兔体重分别为 (5 0 .5± 6 .1) g ,(5 1.6± 4 .3) g ;原免疫状态为雄 (- )雌 (+)抑抗灵低剂量组 ,中剂量组 ,高剂量组新生兔体重分别为 (49.7± 5 .9)g ,(5 1.1± 5 .5 )g ,(5 0 .8± 4 .1)g ;强地松对照组和未治疗组新生兔体重分别 (5 1.6±6 .2 ) g ,(5 1.6± 4 .2 ) g。原免疫状态为雄 (+)雌 (+)抑抗灵低剂量组 。Objective: To observe the effects of Chinese traditional medicine Yi Kang Ling (YKL) on immunity infertility caused by anti sperm antibodies (AsAb). Methods: With the AsAb infertile rabbit as the experimental model, seventy five pairs of New zealand rabbits were divided into three group including YKL treatment group (sub divided into mini , midi and maxi dosage groups), prednisone treatment group and non treatment group. Rabbits of the YKL treatment sub groups were further divided into M + F - (male positive,female negative), M - F + , (male negative, female positive) and M + F + (male positive, female positive)pairs according to their initial AsAb detection results.The control group consisted of five pairs of normal rabbits. When the expected AsAb reversing ratio was achieved, the rabbits were matted and observed for the number of the pregnant and the weight of the newborn.Results: Statistics showed that in M + F - pairs both the midi dosage of YKL and prednisone treatment groups had fertility, in the mini and maxi dosage of YKL treatment groups, 20% of the female rabbits failed to be pregnant, while in the non treatment group, 60% female rabbits remained sterile. The sterile ratios of the M - F + pairs in the mini , midi and maxi dosage of YKL and prednisone treatment groups were 0, 20%, 25% and 25%, respectively, while the sterile ratio in the non treatment M - F + group was 40%. In M + F + groups, the sterile ratios of the three YKL sub groups , prednisone treatment and non treatment groups were 20%, 20% and 60% respectively. In the control group the sterile ratio was 20%. The weight of the newborn rabbits were around 50 grams with no visible malformation. Conclusions: YKL can effectively reverse the AsAb positive results,and restore the fertility of female rabbits. Mini dosage of YKL for 45 days produced the best results and maxi dosage of YKL had no negative effects on the weight of the newborn rabbit.

关 键 词：抗精子抗体 抑抗灵 免疫性不育 

分 类 号：R285.5[医药卫生—中药学] R711.6[医药卫生—中医学]