枸杞多糖对大鼠生殖系统保护作用的机制探讨  

Discussion of Protective Mechanism of Lycium Barbarum Polysaccharides on Reproductive System in Rats

作　　者：黄晓兰[1] 杨明亮[1] 吴晓旻[1] 闫俊[1] 罗琼[1] 

机构地区：[1]武汉大学公共卫生学院,武汉430071

出　　处：《武汉大学学报（医学版）》2004年第1期29-31,41,共4页Medical Journal of Wuhan University

基　　金：湖北省科技厅重点攻关课题 ( 2 0 0 1AA3 0 7B) ;达能膳食营养研究与宣教基金 ( 2 0 0 3 0 5 )

摘　　要：目的 :探讨枸杞多糖 (LBP)对大鼠生殖系统保护作用的可能机制。方法 :36只雄性Wistar大鼠 ,按给予枸杞多糖的不同剂量分组 (0剂量对照组、0剂量阳性对照组、1 0 ,5 0 ,1 0 0 ,2 0 0mg·kg-1 ·d-1 剂量组 ) ,每组 6只。 1 4d后以温水浴构造大鼠睾丸损伤模型 ,测定血清性激素水平、睾丸组织中超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量及观察睾丸组织形态学改变。结果 :大鼠血清性激素水平、SOD活性低于LBP组 ;MDA含量则高于LBP组 ,最佳剂量为LBP 1 0mg·kg-1 ·d-1 ;LBP能降低高温引起的生精细胞损伤、促进睾丸生殖细胞正常发育。结论 :LBP对大鼠生殖系统具有保护作用 ,其机制可能是通过抗氧化作用及调节下丘脑 垂体Objective: To investigate the protective effect of Lycium Barbarum Polysaccharides(LBP) on rat reproductive system. Methods: 36 Wistar male rats were divided into 6 groups according to their different doses of LBP(0 control,0 positive control,10,50,100 and 200 mg·kg -1 ·d -1 ) with 6 rats in each. Trauma model of testicle were constructed by warm bath and serum sex hormone levels, superoxide dismutase(SOD) activity, malonic aldehyde(MDA) contents and morphological change were determined after fourteen days. Results: Serum sex hormone levels and testis SOD activity in positive control group were significantly lower than LBP group. Testis MDA content in positive collate group were significantly higher than LBP group. The optimal dose of LBP was 10 mg·kg -1 ·d -1 . LBP protected the spermatogenic cells and promoted the normal development of testicle germ cells. Conclusion: Lycium Barbarum Polysaccharide can protect rat function of reproductive system which mechanism may be the anti oxidation effect and the adjustment of the hypothalamus pituitary gonadal axis.

关 键 词：枸杞多糖 抗氧化 下丘脑-垂体-性腺轴 大鼠 生殖系统 保护作用 保护机制 中药 

分 类 号：R285.5[医药卫生—中药学]