堆型艾美耳球虫(E.acervulina)单克隆抗体细胞株的建立  

Production of Monoclonal Antibody Cell Strains of E.acervulina

作　　者：王承民[1] 秦建华[1] 赵月兰[2] 冯雪领 郝延刚[1] 李铁栓[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省畜牧兽医研究所,河北保定071000 [3]河北省动物检疫站,河北石家庄050000

出　　处：《中国兽医学报》2004年第1期26-28,共3页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目 ( 3 0 0 113 )

摘　　要：利用细胞融合技术 ,将堆型艾美耳球虫 (Eimeria acervulina)的子孢子可溶性抗原免疫的小鼠脾细胞和 SP2 / 0骨髓瘤细胞融合 ,通过间接 EL ISA方法筛选 ,得到能稳定分泌针对堆型艾美耳球虫子孢子的特异性抗体细胞株 5 B4和5 G7。用间接 EL ISA方法对这 2株细胞的腹水效价进行测定 ,结果 5 G7细胞株的腹水效价为 1∶ 2 6 2 14 4 ,5 B4细胞株的腹水效价为 1∶ 1310 72。腹水经正辛酸 -硫酸铵二步法纯化 ,SDS- PAGE结果表明 ,此方法对 5 G7细胞株纯化效果较好 ,但对 5By means of techniques of cell fusion,the spleen cells of BALB/c immuned with soluble antigen of E.acervulina sporoziotes were fused with SP2/0 myeloma and screened through indirect ELISA,obtained two hybrid cell strains(5B4 and 5G7 cell strains) which could secret specific antibody steadily.Monoclonal antibodies from ascites were purified through combination of the caprilic acid and ammonium sulfate and measured by indirect ELISA with the McAb 5B4 of (1∶(131 072)) and the McAb 5G7 of (1∶(262 144)) respectively.The results of SDS-PAGE showed the way of purification would be suitable for 5G7 cell strain but not for 5B4.

关 键 词：堆型艾美耳球虫 子孢子 单克隆抗体 药物防治 

分 类 号：S852.723[农业科学—基础兽医学]