伊氏锥虫对安锥赛抗药性机制的初步探讨  

Mechanism of Antrycide-resistance of Trypanosoma evansi

作　　者：廖党金[1] 沈杰[2] 

机构地区：[1]四川省畜牧科学研究院,四川成都610066 [2]中国农业科学院上海家畜寄生虫病研究所,上海200232

出　　处：《中国兽医学报》2004年第1期28-30,共3页Chinese Journal of Veterinary Science

摘　　要：选用克隆伊氏锥虫的安锥赛敏感株 C0 1、C0 3和 5个不同程度抗药株 C1、C2、C4、C6、C7,用聚丙烯酰胺凝胶电泳分离它们的己糖激酶 (HK)、6 -磷酸葡萄糖脱氢酶 (G6 PDH)、丙氨酸转氨酶 (AL AT)、天冬氨酸转氨酶 (ASAT)同功酶 ,并测定胆碱酯酶活性。结果 ,这 5种酶与伊氏锥虫对安锥赛产生抗药性无关。用 SDS-聚丙烯酰胺凝胶电泳分离和测定这 7个样品的粗蛋白质 ,结果相对分子质量为 15 790带在抗药株 C1、C2、C4、C6和 C7含量较高 ,这表明它可能与安锥赛抗药性的产生有一定关系 ;相对分子质量为 1976 0带在高抗药株 C4、C6和 C7的含量很高 。In order to know the antrycide-resistance of Trypanosoma evansi,HK,G6PDH,ALAT,ASAT isoenzyme patterns were isolated from each cloned T.evansi line,C01,C03,C1,C2,C4,C6 or C7 using the polyacrylamide gel electrophoresis.The comparison of the relative migration rate and relative activities of the isoenzyme bands revealed that there were not obvious differences among them.The activities of the 7 line′s CHE also revealed that the different activity of the enzyme did not exist among them.Those suggested that the 5 enzymes did not involved in the antrycide-resistance of T.evansi.SDS-polyacrylamide gel electrophoresis was also preformed to isolated protein patterns of the 7 populations.The relative migration rates and relative quantities of the protein bands showed that the bands of (15 790) in the lines with the antrycide-resistance(C1,C2,C4,C6 and C7) and the other protein band of (19 760) in the lines with higher levels of the antrycide-resistance(C4,C6 and C7) might involve in the antrycide-resistance of T.evansi.

关 键 词：伊氏锥虫 安锥赛 抗药性机制 酶 蛋白质 

分 类 号：S852.72[农业科学—基础兽医学]