日本血吸虫中国大陆株Sj23抗原基因在家蚕细胞中的表达及鉴定  

Expression and Identification of Gene Coding for Sj23 Antigen in Bombyx mori Cells

作　　者：肖西志[1] 林矫矫 于三科[1] 张亮 程国峰 顾越星[3] 金亚美 沈阳 苑纯秀 刘金明 付志强 张福来 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]上海动物生物技术研究中心,上海200232 [3]中国农业科学院上海家畜寄生虫病研究所,上海200232 [4]西安亨通光华制药有限公司,陕西西安710075

出　　处：《中国兽医学报》2004年第1期43-46,共4页Chinese Journal of Veterinary Science

基　　金：上海市科技发展基金资助项目 ( 0 0 49190 74)

摘　　要：将日本血吸虫 2 30 0 0膜蛋白 (Sj2 3抗原 )基因从原核表达载体 p ET2 8(c)中亚克隆入转移载体 p Bac PAK- His1,构建了 p Bac PAK- his1- Sj2 3转移载体 ,并与线性化家蚕杆状病毒共转染家蚕细胞 ;经 PCR鉴定得到重组病毒 ,经过蓝、白斑筛选得到纯化的重组病毒 ;SDS- PAGE显示日本血吸虫 2 30 0 0膜蛋白基因在家蚕细胞中实现表达 ,蛋白的相对分子质量为 2 6 0 0 0 ;重组 Sj2 3经 Western-The construction of transfer vector,pBacPAK-His1-Sj23,is made through subcloning the Sj23 membrane protein gene from vector of pET28(c) into the transfer vector of pBacPAK-His1.The transfer vector pBacPaK-his1-Sj23 then co-trans-fected Bombyx mori cells with linealized virus of Bombyx mori nuclear polyhedrosis virus.The recombinant virus was got through PCR identification and the purified recombinant virus was got by blue and white plaque assay.SDS-PAGE shows that the (23 000) membrane protein was expressed in Bombyx mori cells,and the molecular weight is (26 000).The recombinant Sj23 can be recognized by the serum of rabbit suffered from the S.japonicum.

关 键 词：日本血吸虫 Sj23抗原基因 家蚕细胞 原核表达 

分 类 号：S852.735[农业科学—基础兽医学] Q786[农业科学—兽医学]