金叶败毒治疗孕早期豚鼠巨细胞病毒感染的研究  

A Study on Traditional Chinese Medicine Jingyebaidu for Treating Anti-early Pregnancy Infection with Guinea Pig Cytomegalovirus

作　　者：陈素华[1] 熊锦文[1] 邢玮[1] 赵婕[1] 刘海智[1] 王昕荣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《医药导报》2004年第1期1-3,共3页Herald of Medicine

基　　金：国家自然科学基金资助项目 (基金编号 :3 9970 769)

摘　　要：目的 :探讨清热解毒中药金叶败毒抗孕早期豚鼠巨细胞病毒 (guineapigcytomegalovirus ,GPCMV)感染的效果及孕期用药的安全性。方法 :①随机选择妊娠早期雌豚鼠 75只分成 3组 ,每组 2 5只。空白对照组 :不接种病毒。阳性对照组 :每只腹腔接种GPCMV悬液 1mL(10 7TCID50 )。治疗组 :按阳性对照组接种病毒 ,同时灌胃给予中药金叶败毒 (tid ,连用 14d)。 2 0d后处死豚鼠 ,采集血液及各脏器组织 ,检测感染情况。②随机选取受孕后第 1～ 18天 (小鼠器官发生期 )的雌鼠 90只 ,随机分成 3组 ,每组 3 0只。空白对照组不给药。阳性对照组给予致畸阳性对照药维生素A ,10 0mg·kg 1 ·d 1 ,每日分 3次灌胃给药。治疗组根据小鼠灌胃最大容积给予金叶败毒 ,将 0 .5g药溶于 3 7℃温开水 0 .5mL ,tid ,连用 18d。追踪母鼠一般情况及胎仔生长发育状况。结果 :①阳性对照组和治疗组的血液GPCMVDNA阳性率分别为 10 0 .0 % (2 5 2 5 )和 44 .0 % (11 2 5 ) ,差异有极显著性 (χ2 =3 .94,P <0 .0 1) ,阳性对照组出现被毛耸立等感染征象 96.0 %(2 4 2 5 ) ,死亡 4.0 % (1 2 5 ) ,治疗组无任何感染征象。②小鼠器官发生期给药不增加胎仔生长发育异常的风险。结论 :中药金叶败毒具有抗孕早期GPCMV感染的效果 ,孕期用药比较安全。Objective:To investigate the effect of traditional Chinese medicine jinyebaidu on early pregnant guinea pigs infected with guinea pig cytomegalovirus (GPCMV) and its safety in pregnancy. Methods:①A total of 75 pregnant guinea pigs in the first trimester were randomly divided into three groups: blank control group were treated with nothing special; positive control group were only inoculated with 1ml suspension of GPCMV intraperioneally; treatment group were given jinyebaidu through stomach perfusing after being inoculated. The guinea pigs were killed after 20 days and the GPCMV infection rate was examined in the blood and organs ②90 pregnant mice were randomly divided into three groups: blank control group were given nothing; positive control group were treated with vitamin A and treatment group accepted jinyebaidu. The effect on pregnant mice and the development of their offsprings were followed up. Results:①the GPCMV DNA positive rate of blood in the treatment group was lower than that of positive group(χ2=39.4, P<0.01) significantly. The rates of viremia manifestation and death were 96.0%(24/25) and 4.0% (1/25) respectively in the positive group, but no infection sign was seen in the treatment group. ②Jinyebaidu has no effect on the offsprings. Conclusion:Traditional Chinese medicine jinyebaidu markedly reduces the GPCMV maternal infection rate. The medicine is safe when used in pregnant mice.

关 键 词：金叶败毒 巨细胞病毒 豚鼠 妊娠 

分 类 号：R285.5[医药卫生—中药学]